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Literature summary extracted from

  • Barsky, D.L.; Hoffee, P.A.
    Purification and characterization of phosphopentomutase from rat liver (1983), Biochim. Biophys. Acta, 743, 162-171.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.7 Cys activates Rattus norvegicus
5.4.2.7 glucose 1,6-diphosphate activates, optimal concentration 0.01 mM Rattus norvegicus

General Stability

EC Number General Stability Organism
5.4.2.7 glucose 1,6-diphosphate protects against thermal inactivation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.7 AsO43-
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.7 0.083
-
 deoxyribose 1-phosphate
-
 Rattus norvegicus
5.4.2.7 0.13
-
 ribose 1-phosphate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.7 Mg2+ stimulates Rattus norvegicus
5.4.2.7 Mg2+ optimal concentration 0.2 mM Rattus norvegicus
5.4.2.7 Mn2+ stimulates, optimal concentration is 0.2 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.7 32500
-
 2 * 32500, SDS-PAGE Rattus norvegicus
5.4.2.7 65000
-
 disc gel electrophoresis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.7 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.7
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.7 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.7 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
5.4.2.7 4°C, Tris buffer or phosphate buffer, pH 7-8, stable for 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.7 D-ribose 1-phosphate
-
 Rattus norvegicus D-ribose 5-phosphate
-
 ?
5.4.2.7 Deoxyribose 1-phosphate at 25% of the activity relative to ribose 1-phosphate Rattus norvegicus Deoxyribose 5-phosphate
-
 ?
5.4.2.7 Glucose 1-phosphate at 2.4% of the activity relative to ribose 1-phosphate Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.2.7 dimer 2 * 32500, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.7 50
-
 pH 8, rapid loss of activity in absence of glucose 1,6-diphosphate, 70% loss of activity after 10 min, complete loss of activity after 1 h. Completely stable for 90 min in presence of 11 mM glucose 1,6-diphosphate Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.7 7.5 8.5
-
 Rattus norvegicus