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Literature summary extracted from

  • Schauer, R.; Wember, M.
    Isolation and characterization of sialate lyase from pig kidney (1996), Biol. Chem. Hoppe-Seyler, 377, 293-299.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.3 5,5-dithiobis(2-nitrobenzoic acid) 50% residual activity at 0.3 mg/ml Sus scrofa
4.1.3.3 5-Diazonium-1-H-tetrazole 20% residual activity at 5 mM Sus scrofa
4.1.3.3 CN- 50% residual activity at 20 mM Sus scrofa
4.1.3.3 diethyldicarbonate 50% residual activity at 0.1% Sus scrofa
4.1.3.3 NaBH4
-
 Sus scrofa
4.1.3.3 o-phenanthroline 40% residual activity at 0.1 mM Sus scrofa
4.1.3.3 p-chloromercuribenzoate 70% residual activity at 0.1 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 3.7
-
 N-acetylneuraminic acid
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.3 55000
-
 gel filtration Sus scrofa
4.1.3.3 61000
-
 SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Sus scrofa
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.3.3 sensitive to illumination in presence of Rose Bengal Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.3 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.3 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 additional information not: N-acetyl-4-O-acetylneuraminic acid and 2-deoxy-2,3-didehydro-N-acetylneuraminic acid Sus scrofa ?
-
 ?
4.1.3.3 N-Acetyl-9-O-acetylneuraminic acid
-
 Sus scrofa ?
-
 ?
4.1.3.3 N-Acetylneuraminic acid
-
 Sus scrofa N-Acetyl-D-mannosamine + pyruvate
-
 ?
4.1.3.3 N-Glycolylneuraminic acid
-
 Sus scrofa ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.3 7.2
-
-
 Sus scrofa