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Literature summary extracted from

  • Mann, D.F.; Floss, H.G.
    Purification and properties of anthranilate synthetase from the ergot fungus, Claviceps spec., strain SD 58 (1977), Lloydia, 40, 136-145.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.3.27 anthranilate synthetase activity of the enzyme complex is quite unstable unless Gln, MgCl2, Tris and glycerol are included in the extraction buffer Claviceps sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 elymoclavine i.e. (6-methyl-8,9-didehydroergolin-8yl)methanol Claviceps sp.
4.1.3.27 L-Trp
-
 Claviceps sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.009
-
 chorismate
-
 Claviceps sp.
4.1.3.27 10
-
 Gln
-
 Claviceps sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 200000
-
 three-enzyme complex containing anthranilate synthetase, phosphoribosyl anthranilate isomerase and indole-3-glycerol phosphate synthetase, sucrose density gradient centrifugation Claviceps sp.
4.1.3.27 400000
-
 three-enzyme complex containing anthranilate synthetase, phosphoribosyl anthranilate isomerase and indole-3-glycerol phosphate synthetase, gel filtration Claviceps sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Claviceps sp.
-
-
-
4.1.3.27 Claviceps sp.
-
 three-enzyme complex containing anthranilate synthetase, phosphoribosyl anthranilate isomerase and indole-3-glycerol phosphate synthetase
-
4.1.3.27 Claviceps sp. SD58
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27 partial Claviceps sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.27 mycelium
-
 Claviceps sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.27 0.585
-
-
 Claviceps sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Claviceps sp. anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + L-Gln
-
 Claviceps sp. SD58 anthranilate + pyruvate + L-glutamate
-
 ?