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Literature summary extracted from

  • Schmauder, H.P.; Gr๖ger, D.
    Zur Charakterisierung der Anthranilat-Synthetase bei Claviceps (1976), Biochem. Physiol. Pflanz., 169, 471-486.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.27 chanoclavine slight activation in presence of His Claviceps sp.
4.1.3.27 elymoclavine slight activation in presence of His Claviceps sp.
4.1.3.27 indoleacrylic acid slight activation in presence of His Claviceps sp.
4.1.3.27 prephenic acid slight activation in presence of His Claviceps sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 Ba2+
-
Claviceps sp.
4.1.3.27 Ca2+
-
Claviceps sp.
4.1.3.27 Fe2+
-
Claviceps sp.
4.1.3.27 Hg2+ 0.005 mM HgCl2, complete inhibition Claviceps sp.
4.1.3.27 iodoacetamide
-
Claviceps sp.
4.1.3.27 L-Trp
-
Claviceps sp.
4.1.3.27 Pb2+
-
Claviceps sp.
4.1.3.27 PCMB 0.005 mM, complete inhibition Claviceps sp.
4.1.3.27 Zn2+ 1 mM, 75% inhibition Claviceps sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.04
-
chorismate
-
Claviceps sp.
4.1.3.27 4
-
Gln
-
Claviceps sp.
4.1.3.27 8 9 NH4+
-
Claviceps sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Co2+
-
Claviceps sp.
4.1.3.27 K+ stimulates Claviceps sp.
4.1.3.27 Mg2+ required Claviceps sp.
4.1.3.27 Mg2+ Km: 0.4 mM Claviceps sp.
4.1.3.27 Mn2+
-
Claviceps sp.
4.1.3.27 Na+ stimulates Claviceps sp.
4.1.3.27 NH4+ stimulates Claviceps sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Claviceps purpurea
-
strain Pepty 695 and strain M5/95F
-
4.1.3.27 Claviceps sp.
-
-
-
4.1.3.27 Claviceps sp. SD58
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27 partial Claviceps sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
Claviceps purpurea anthranilate + pyruvate + L-glutamate
-
?
4.1.3.27 chorismate + L-Gln
-
Claviceps sp. anthranilate + pyruvate + L-glutamate
-
?
4.1.3.27 chorismate + L-Gln
-
Claviceps sp. SD58 anthranilate + pyruvate + L-glutamate
-
?
4.1.3.27 chorismate + NH4+
-
Claviceps sp. ?
-
?
4.1.3.27 chorismate + NH4+
-
Claviceps sp. SD58 ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 7.8
-
Gln-dependent activity Claviceps sp.
4.1.3.27 8.6
-
NH4+-dependent activity Claviceps sp.