Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tack, B.F.; Chapman, P.J.; Dagley, S.
    Purification and properties of 4-hydroxy-4-methyl-2-oxoglutarate aldolase (1972), J. Biol. Chem., 247, 6444-6449.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.17 oxaloacetate competitive Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.17 0.067
-
 4-hydroxy-4-methyl-2-oxoglutarate
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.17 Mg2+ Mn2+ or Mg2+ required Pseudomonas putida
4.1.3.17 Mg2+ Km: 0.17 mM MgCl2 Pseudomonas putida
4.1.3.17 Mn2+ Mn2+ or Mg2+ required Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.17 27000
-
 6 * 27000, SDS-PAGE Pseudomonas putida
4.1.3.17 150000
-
 equilibrium sedimentation Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.17 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.17
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.17 4-carboxy-4-hydroxy-2-oxoadipate
-
 Pseudomonas putida pyruvate + oxaloacetate
-
 ?
4.1.3.17 4-hydroxy-4-methyl-2-oxoglutarate the enzyme attacks only one enantiomer Pseudomonas putida pyruvate + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.17 hexamer 6 * 27000, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.17 8
-
-
 Pseudomonas putida