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Literature summary extracted from

  • Jameel, S.; El-Gul, T.; McFadden, B.A.
    Isolation and properties of watermelon isocitrate lyase (1984), Phytochemistry, 23, 2753-2759.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.1 2-mercaptoethanol increase of activity Citrullus lanatus subsp. vulgaris
4.1.3.1 dithiothreitol increase of activity Citrullus lanatus subsp. vulgaris
4.1.3.1 dithiothreitol optimal concentration 5 mM Citrullus lanatus subsp. vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 2-mercaptoethanol increase of activity Citrullus lanatus subsp. vulgaris
4.1.3.1 oxalate
-
 Citrullus lanatus subsp. vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.25
-
 Ds-isocitrate
-
 Citrullus lanatus subsp. vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 64000
-
 4 * 64000, SDS-PAGE Citrullus lanatus subsp. vulgaris
4.1.3.1 277000
-
 gel filtration Citrullus lanatus subsp. vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Citrullus lanatus subsp. vulgaris glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Citrullus lanatus subsp. vulgaris
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.3.1 no modification non-covalently bound carbohydrate Citrullus lanatus subsp. vulgaris

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Citrullus lanatus subsp. vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 16.1
-
-
 Citrullus lanatus subsp. vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Citrullus lanatus subsp. vulgaris succinate + glyoxylate
-
 r
4.1.3.1 isocitrate glyoxylate cycle Citrullus lanatus subsp. vulgaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer 4 * 64000, SDS-PAGE Citrullus lanatus subsp. vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.1 additional information
-
 oxalate protects Citrullus lanatus subsp. vulgaris