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Literature summary extracted from

  • Achar, B.S.; Vaidyanathan, C.S.
    Purification and properties of uridine hydrolase from mung-bean (Phaseolus radiatus) seedlings (1967), Arch. Biochem. Biophys., 119, 356-362.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.2.3 enzyme retains about 95% of the original activity after dialysis against 0.01 M Tris-HCl buffer, pH 7.5 for 24 h Vigna radiata var. radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.3 Ag+
-
 Vigna radiata var. radiata
3.2.2.3 Co2+
-
 Vigna radiata var. radiata
3.2.2.3 NEM
-
 Vigna radiata var. radiata
3.2.2.3 PCMB reversal by sulfhydryl agents Vigna radiata var. radiata
3.2.2.3 Zn2+
-
 Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.3 1
-
 uridine
-
 Vigna radiata var. radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.3 additional information the enzyme does not require metal ions for activity Vigna radiata var. radiata

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.3 Vigna radiata var. radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.3
-
 Vigna radiata var. radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.3 seedling
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.3 0.725
-
-
 Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.3 uridine + H2O
-
 Vigna radiata var. radiata uracil + D-ribose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.3 45
-
-
 Vigna radiata var. radiata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.3 30 60 30°C: about 60% of maximal activity, 60°C: about 35% of maximal activity Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.3 7.4
-
-
 Vigna radiata var. radiata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.3 6.8 8 pH 6.8: about 70% of maximal activity, pH 8.0: about 65% of maximal activity Vigna radiata var. radiata