BRENDA - Enzyme Database

Bakers yeast uridine nucleosidase. Purification, composition, and physical and enzymatic properties

Magni, G.; Fioretti, E.; Ipata, P.L.; Natalini, P.; J. Biol. Chem. 250, 9-13 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.3
D-ribose
-
Saccharomyces cerevisiae
3.2.2.3
glucose 6-phosphate
-
Saccharomyces cerevisiae
3.2.2.3
ribosylthymine
competitive
Saccharomyces cerevisiae
3.2.2.3
Tris
twice recrystallized, 30 mM, 50% inhibition
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.3
0.86
-
uridine
-
Saccharomyces cerevisiae
3.2.2.3
1.66
-
5-methyluridine
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.3
17000
-
2 * 17000, SDS-PAGE
Saccharomyces cerevisiae
3.2.2.3
32500
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.3
Saccharomyces cerevisiae
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.3
1.56
-
-
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
3.2.2.3
-20°C, stable for several months
Saccharomyces cerevisiae
3.2.2.3
4°C, 100 mM phosphate buffer, pH 7.2, 50 mM Tris-HCl, pH 7.0, stable for up to 15 days
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.3
5-methyluridine + H2O
-
326398
Saccharomyces cerevisiae
5-methyluracil + D-ribose
-
-
-
?
3.2.2.3
uridine + H2O
-
326398
Saccharomyces cerevisiae
uracil + D-ribose
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.2.3
dimer
2 * 17000, SDS-PAGE
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.3
7
-
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.3
D-ribose
-
Saccharomyces cerevisiae
3.2.2.3
glucose 6-phosphate
-
Saccharomyces cerevisiae
3.2.2.3
ribosylthymine
competitive
Saccharomyces cerevisiae
3.2.2.3
Tris
twice recrystallized, 30 mM, 50% inhibition
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.3
0.86
-
uridine
-
Saccharomyces cerevisiae
3.2.2.3
1.66
-
5-methyluridine
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.3
17000
-
2 * 17000, SDS-PAGE
Saccharomyces cerevisiae
3.2.2.3
32500
-
gel filtration
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.3
1.56
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.2.3
-20°C, stable for several months
Saccharomyces cerevisiae
3.2.2.3
4°C, 100 mM phosphate buffer, pH 7.2, 50 mM Tris-HCl, pH 7.0, stable for up to 15 days
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.3
5-methyluridine + H2O
-
326398
Saccharomyces cerevisiae
5-methyluracil + D-ribose
-
-
-
?
3.2.2.3
uridine + H2O
-
326398
Saccharomyces cerevisiae
uracil + D-ribose
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.2.3
dimer
2 * 17000, SDS-PAGE
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.3
7
-
-
Saccharomyces cerevisiae