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Literature summary extracted from

  • Magni, G.
    Uridine nucleosidase from yeast (1978), Methods Enzymol., 51, 290-296.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.3 1,10-phenanthroline
-
 Saccharomyces cerevisiae
3.2.2.3 CdCl2
-
 Saccharomyces cerevisiae
3.2.2.3 Co2+ CoCl2 Saccharomyces cerevisiae
3.2.2.3 CuCl2
-
 Saccharomyces cerevisiae
3.2.2.3 D-ribose competitive Saccharomyces cerevisiae
3.2.2.3 EDTA
-
 Saccharomyces cerevisiae
3.2.2.3 glucose 6-phosphate competitive Saccharomyces cerevisiae
3.2.2.3 MnCl2
-
 Saccharomyces cerevisiae
3.2.2.3 NaCN
-
 Saccharomyces cerevisiae
3.2.2.3 NiCl2
-
 Saccharomyces cerevisiae
3.2.2.3 ribose 5-phosphate
-
 Saccharomyces cerevisiae
3.2.2.3 ribosylthymine competitive Saccharomyces cerevisiae
3.2.2.3 ribulose 5-phosphate
-
 Saccharomyces cerevisiae
3.2.2.3 ZnCl2
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.3 0.86
-
 uridine
-
 Saccharomyces cerevisiae
3.2.2.3 1.66
-
 5-methyluridine
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.3 Cu2+ contains 1 gatom of Cu2+ per enzyme molecule Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.3 16500
-
 2 * 16500, SDS-PAGE Saccharomyces cerevisiae
3.2.2.3 32500
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.3 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.3 1.56
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.2.2.3 -20°C, stable for several months Saccharomyces cerevisiae
3.2.2.3 4°C, 100 mM phosphate buffer, pH 7.2, 50 mM Tris-HCl, pH 7.0, stable for up to 15 days Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.3 5-methyluridine + H2O
-
 Saccharomyces cerevisiae 5-methyluracil + D-ribose
-
 ?
3.2.2.3 uridine + H2O
-
 Saccharomyces cerevisiae uracil + D-ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.3 dimer 2 * 16500, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.3 7.1
-
-
 Saccharomyces cerevisiae