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Literature summary extracted from

  • Hassan, M.M.; El-Zainy, T.A.; Alla, A.M.
    Studies of some of the properties of purine nucleoside hydrolase from Aspergillus niger (1979), Egypt. J. Chem., 22, 189-196.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.1 2-mercaptoethanol increases activity Aspergillus niger
3.2.2.1 GSH increases activity Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.1 EDTA 0.01 M, 28% inhibition. 0.02 M 42% inhibition Aspergillus niger
3.2.2.1 HgCl2
-
 Aspergillus niger
3.2.2.1 iodoacetate 0.01 M, 25% inhibition. 0.02 M 36.1% inhibition Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.1 4.1
-
 Inosine
-
 Aspergillus niger
3.2.2.1 7.5
-
 guanosine
-
 Aspergillus niger
3.2.2.1 9
-
 adenosine
-
 Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.1 Mg2+ increases activity Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.1 4
-
-
 Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
3.2.2.1 4°C, 0.1 M citrate buffer, pH 4.0, stable for more than 3 months Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 adenosine + H2O
-
 Aspergillus niger D-ribose + adenine
-
 ?
3.2.2.1 guanosine + H2O
-
 Aspergillus niger D-ribose + guanine
-
 ?
3.2.2.1 inosine + H2O
-
 Aspergillus niger hypoxanthine + D-ribose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.1 60
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.1 30 70 30°C: about 35% of maximal activity, 70°C: about 20% of maximal activity Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.1 60
-
 60 min, complete loss of activity. 30 min, 30% loss of activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.1 3 4
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.1 1.5 5.3 about 50% of maximal activity at pH 1.5 and at pH 5.3 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.1 8
-
 unstale Aspergillus niger