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Literature summary extracted from

  • Atkins, C.A.; Shelp, B.J.; Storer, P.J.
    Purification and properties of purine nucleosidase from N2-fixing nodules of cowpea (Vigna unguiculata) (1989), J. Plant Physiol., 134, 447-452.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.1 adenosine competitive inhibitor of xanthosine hydrolysis and inosine hydrolysis Vigna unguiculata
3.2.2.1 guanosine competitive inhibitor of xanthosine hydrolysis Vigna unguiculata
3.2.2.1 Inosine competitive inhibitor of xanthosine hydrolysis Vigna unguiculata
3.2.2.1 uridine competitive inhibitor of xanthosine hydrolysis Vigna unguiculata
3.2.2.1 Xanthosine competitive inhibitor of inosine hydrolysis Vigna unguiculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.1 0.8
-
 Xanthosine
-
 Vigna unguiculata
3.2.2.1 0.83
-
 Inosine
-
 Vigna unguiculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.1 30600
-
 5 * 30600, SDS-PAGE Vigna unguiculata
3.2.2.1 160000
-
 gel filtration Vigna unguiculata

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Vigna unguiculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1
-
 Vigna unguiculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.1 nodule N2-fixing, nodulated with Rhizobium strain B 756 Vigna unguiculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.1 0.432
-
-
 Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 adenosine + H2O 3.6% of the activity with xanthosine Vigna unguiculata D-ribose + adenine
-
 ?
3.2.2.1 cytidine + H2O
-
 Vigna unguiculata D-ribose + cytosine
-
 ?
3.2.2.1 guanosine + H2O 1.4% of the activity with xanthosine Vigna unguiculata D-ribose + guanine
-
 ?
3.2.2.1 inosine + H2O 25% of the activity with xanthosine Vigna unguiculata hypoxanthine + D-ribose
-
 ?
3.2.2.1 thymidine + H2O
-
 Vigna unguiculata D-ribose + thymine
-
 ?
3.2.2.1 uridine + H2O
-
 Vigna unguiculata D-ribose + uracil
-
 ?
3.2.2.1 xanthosine + H2O
-
 Vigna unguiculata D-ribose + xanthine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.1 pentamer 5 * 30600, SDS-PAGE Vigna unguiculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.1 7.5 8.5 hydrolysis of inosine and xanthosine Vigna unguiculata