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Literature summary extracted from

  • Yoshioka, H.; Hayashida, S.
    Production and purification of thermostable beta-glucosidase from Mucor miehei YH-10 (1980), Agric. Biol. Chem., 44, 2817-2824.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Rhizomucor miehei
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Rhizomucor miehei cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Rhizomucor miehei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography techniques, 2 isoforms Rhizomucor miehei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture maximal enzyme synthesis, if cells grown in wheat bran medium Rhizomucor miehei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Rhizomucor miehei beta-D-glucose + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
-
 Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 70
-
 isoform G-a, stable for 5 min Rhizomucor miehei
3.2.1.21 80
-
 isoform G-b, completely inactivated after 5 min Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 8
-
-
 Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 2.5 10 stable within for 24 h at 4°C Rhizomucor miehei