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Literature summary extracted from

  • Ait, N.; Creuzet, N.; Cattaneo, J.
    Properties of beta-glucosidase purified from Clostridium thermocellum (1982), J. Gen. Microbiol., 128, 569-577.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 beta-D-glucose competitive inhibition, Ki: 135 mM Acetivibrio thermocellus
3.2.1.21 D-glucono-1,5-lactone competitive inhibition, Ki: 0.01 mM Acetivibrio thermocellus
3.2.1.21 Hg2+ complete inactivation at 0.1 mM Acetivibrio thermocellus
3.2.1.21 iodoacetoamide complete inhibition at 10 mM Acetivibrio thermocellus
3.2.1.21 p-chloromercuribenzoic acid 53% inhibition at 0.5 mM Acetivibrio thermocellus
3.2.1.21 Tris 54% inhibition at 10 mM Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 2.6
-
4-nitrophenyl-beta-D-glucopyranoside
-
Acetivibrio thermocellus
3.2.1.21 83
-
cellobiose
-
Acetivibrio thermocellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 cell associated
-
Acetivibrio thermocellus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 43000
-
gel filtration Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acetivibrio thermocellus low activity beta-D-glucose + D-glucose
-
?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acetivibrio thermocellus sophorose beta-D-glucose + D-glucose
-
?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acetivibrio thermocellus laminaribiose beta-D-glucose + D-glucose
-
?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Acetivibrio thermocellus low activity beta-D-galactose + D-glucose
-
?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Acetivibrio thermocellus lactose beta-D-galactose + D-glucose
-
?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Acetivibrio thermocellus cellobiose beta-D-glucose + D-glucose
-
?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Acetivibrio thermocellus low activity beta-D-glucose + D-glucose
-
?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Acetivibrio thermocellus gentiobiose beta-D-glucose + D-glucose
-
?
3.2.1.21 cellopentaose + 4 H2O Acetivibrio thermocellus
-
5 D-glucose
-
?
3.2.1.21 cellotetraose + 3 H2O Acetivibrio thermocellus
-
4 D-glucose
-
?
3.2.1.21 cellotriose + 2 H2O Acetivibrio thermocellus
-
3 D-glucose
-
?
3.2.1.21 laminaritriose + 2 H2O Acetivibrio thermocellus
-
3 D-glucose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Acetivibrio thermocellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography techniques Acetivibrio thermocellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
Acetivibrio thermocellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 127
-
-
Acetivibrio thermocellus

Storage Stability

EC Number Storage Stability Organism
3.2.1.21 4°C or -20°C without loss of activity for 1 year Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Acetivibrio thermocellus 2-hydroxymethylphenol + beta-D-glucose
-
?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O low activity Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + beta-D-galactose
-
?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + D-glucopyranose
-
?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + beta-D-xylose
-
?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O low activity Acetivibrio thermocellus beta-D-galactose + D-glucose
-
?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Acetivibrio thermocellus beta-D-galactose + D-glucose
-
?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
3.2.1.21 cellobiose + H2O
-
Acetivibrio thermocellus 2 beta-D-glucose
-
?
3.2.1.21 cellopentaose + 4 H2O
-
Acetivibrio thermocellus 5 D-glucose
-
?
3.2.1.21 cellotetraose + 3 H2O
-
Acetivibrio thermocellus 4 D-glucose
-
?
3.2.1.21 cellotriose + 2 H2O
-
Acetivibrio thermocellus 3 D-glucose
-
?
3.2.1.21 laminaritriose + 2 H2O
-
Acetivibrio thermocellus 3 D-glucose
-
?
3.2.1.21 phenyl-beta-D-glucoside + H2O low activity Acetivibrio thermocellus phenol + beta-D-glucose
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 68
-
half-life: 62.5 min Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6 6.5
-
Acetivibrio thermocellus