EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | beta-D-glucose | competitive inhibition, Ki: 135 mM | Acetivibrio thermocellus | |
3.2.1.21 | D-glucono-1,5-lactone | competitive inhibition, Ki: 0.01 mM | Acetivibrio thermocellus | |
3.2.1.21 | Hg2+ | complete inactivation at 0.1 mM | Acetivibrio thermocellus | |
3.2.1.21 | iodoacetoamide | complete inhibition at 10 mM | Acetivibrio thermocellus | |
3.2.1.21 | p-chloromercuribenzoic acid | 53% inhibition at 0.5 mM | Acetivibrio thermocellus | |
3.2.1.21 | Tris | 54% inhibition at 10 mM | Acetivibrio thermocellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 2.6 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Acetivibrio thermocellus | |
3.2.1.21 | 83 | - |
cellobiose | - |
Acetivibrio thermocellus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.21 | cell associated | - |
Acetivibrio thermocellus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 43000 | - |
gel filtration | Acetivibrio thermocellus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Acetivibrio thermocellus | low activity | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Acetivibrio thermocellus | sophorose | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Acetivibrio thermocellus | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | low activity | beta-D-galactose + D-glucose | - |
? | |
3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | lactose | beta-D-galactose + D-glucose | - |
? | |
3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | low activity | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | cellopentaose + 4 H2O | Acetivibrio thermocellus | - |
5 D-glucose | - |
? | |
3.2.1.21 | cellotetraose + 3 H2O | Acetivibrio thermocellus | - |
4 D-glucose | - |
? | |
3.2.1.21 | cellotriose + 2 H2O | Acetivibrio thermocellus | - |
3 D-glucose | - |
? | |
3.2.1.21 | laminaritriose + 2 H2O | Acetivibrio thermocellus | - |
3 D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Acetivibrio thermocellus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | to homogeneity, chromatography techniques | Acetivibrio thermocellus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.21 | cell culture | - |
Acetivibrio thermocellus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 127 | - |
- |
Acetivibrio thermocellus |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.21 | 4°C or -20°C without loss of activity for 1 year | Acetivibrio thermocellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Acetivibrio thermocellus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | low activity | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-galactoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + beta-D-xylose | - |
? | |
3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | low activity | Acetivibrio thermocellus | beta-D-galactose + D-glucose | - |
? | |
3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Acetivibrio thermocellus | beta-D-galactose + D-glucose | - |
? | |
3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Acetivibrio thermocellus | 2 beta-D-glucose | - |
? | |
3.2.1.21 | cellopentaose + 4 H2O | - |
Acetivibrio thermocellus | 5 D-glucose | - |
? | |
3.2.1.21 | cellotetraose + 3 H2O | - |
Acetivibrio thermocellus | 4 D-glucose | - |
? | |
3.2.1.21 | cellotriose + 2 H2O | - |
Acetivibrio thermocellus | 3 D-glucose | - |
? | |
3.2.1.21 | laminaritriose + 2 H2O | - |
Acetivibrio thermocellus | 3 D-glucose | - |
? | |
3.2.1.21 | phenyl-beta-D-glucoside + H2O | low activity | Acetivibrio thermocellus | phenol + beta-D-glucose | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 68 | - |
half-life: 62.5 min | Acetivibrio thermocellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 6 | 6.5 | - |
Acetivibrio thermocellus |