Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ohmiya, K.; Shirai, M.; Kurachi, Y.; Shimizu, S.
    Isolation and properties of beta-glucosidase from Ruminococcus albus (1985), J. Bacteriol., 161, 432-434.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 2-mercaptoethanol slight activation at 1 mM Ruminococcus albus
3.2.1.21 Cysteine hydrochloride slight activation at 1 mM Ruminococcus albus
3.2.1.21 dithiothreitol slight activation at 1 mM Ruminococcus albus
3.2.1.21 GSH slight activation at 1 mM Ruminococcus albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cu2+ remarkably inhibition at 1 mM Ruminococcus albus
3.2.1.21 Hg2+ remarkable inhibition at 1 mM Ruminococcus albus
3.2.1.21 iodoacetoamide strong inhibition at 1 mM Ruminococcus albus
3.2.1.21 p-chloromercuribenzoic acid strong inhibition at 1 mM Ruminococcus albus
3.2.1.21 Zn2+ remarkable inhibition at 1 mM Ruminococcus albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 2.2
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Ruminococcus albus
3.2.1.21 26
-
 cellobiose
-
 Ruminococcus albus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 cell associated
-
 Ruminococcus albus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 82000
-
 gel filtration, SDS-PAGE Ruminococcus albus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Ruminococcus albus cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O Ruminococcus albus
-
 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O Ruminococcus albus
-
 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O Ruminococcus albus
-
 3 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Ruminococcus albus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein 12% carbohydrate content Ruminococcus albus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography steps Ruminococcus albus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Ruminococcus albus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 13
-
-
 Ruminococcus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-cellobioside + 2 H2O cleaves beta-glucosidic linkages one by one at the non-reducing end only Ruminococcus albus 4-nitrophenol + 2 beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Ruminococcus albus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Ruminococcus albus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Ruminococcus albus 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O
-
 Ruminococcus albus 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Ruminococcus albus 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O
-
 Ruminococcus albus 3 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 82000, SDS-PAGE Ruminococcus albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 30 35
-
 Ruminococcus albus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 37
-
 stable below Ruminococcus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.5
-
-
 Ruminococcus albus