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Literature summary extracted from
- Cloning, sequencing, and characterization of a membrane-associated Prevotella ruminicola B14 beta-glucosidase with cellodextrinase and cyanoglycosidase activities (1995), J. Bacteriol., 177, 5884-5890.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | expression in Escherichia coli | Prevotella ruminicola |
| 3.2.1.74 | Escherichia coli | Prevotella ruminicola |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.74 | 0.42 | - |
p-nitrophenyl-beta-D-glucoside | - |
Prevotella ruminicola |  |
| 3.2.1.74 | 5 | - |
arbutin | - |
Prevotella ruminicola | |
| 3.2.1.74 | 10.7 | - |
gentiobiose | - |
Prevotella ruminicola | |
| 3.2.1.74 | 13.5 | - |
cellobiose | - |
Prevotella ruminicola | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | membrane | extracellular localization | Prevotella ruminicola | 16020 | - |
| 3.2.1.74 | membrane | - |
Prevotella ruminicola | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 87000 | - |
x * 87000, SDS-PAGE | Prevotella ruminicola |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Prevotella ruminicola | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Prevotella ruminicola | less active | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Prevotella ruminicola | low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Prevotella ruminicola | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellodextrin + H2O | Prevotella ruminicola | highest activity | beta-D-glucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Prevotella ruminicola | - |
- |
- |
| 3.2.1.74 | Prevotella ruminicola | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Prevotella ruminicola | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Prevotella ruminicola | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.21 | (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | contribution to cyanide toxicity in ruminants | Prevotella ruminicola | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.21 | (R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O | less active | Prevotella ruminicola | HCN + ? | - |
? | |
| 3.2.1.21 | (R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O | amygdalin | Prevotella ruminicola | HCN + ? | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Prevotella ruminicola | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | less active | Prevotella ruminicola | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Prevotella ruminicola | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Prevotella ruminicola | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellodextrin + H2O | highest activity | Prevotella ruminicola | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | prunasin + H2O | - |
Prevotella ruminicola | D-mandelonitrile + beta-D-glucose | - |
? | |
| 3.2.1.74 | amygdalin + H2O | - |
Prevotella ruminicola | ? | - |
? | |
| 3.2.1.74 | arbutin + H2O | - |
Prevotella ruminicola | ? | - |
? | |
| 3.2.1.74 | cellobiose + H2O | - |
Prevotella ruminicola | D-glucose + cellotetraose + cellotriose | - |
? | |
| 3.2.1.74 | cellopentaose + H2O | - |
Prevotella ruminicola | D-glucose + ? | - |
? | |
| 3.2.1.74 | cellotetraose + H2O | - |
Prevotella ruminicola | D-glucose + cellotriose + cellobiose | - |
? | |
| 3.2.1.74 | cellotriose + H2O | - |
Prevotella ruminicola | D-glucose + cellobiose | - |
? | |
| 3.2.1.74 | gentiobiose + H2O | - |
Prevotella ruminicola | ? | - |
? | |
| 3.2.1.74 | p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Prevotella ruminicola | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.74 | prunasin + H2O | - |
Prevotella ruminicola | D-mandelonitrile + beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.74 | ? | x * 87000, SDS-PAGE | Prevotella ruminicola |
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