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Literature summary extracted from
- Purification, characterization, and substrate specificity of a novel highly glucose-tolerant beta-glucosidase from Aspergillus oryzae (1998), Appl. Environ. Microbiol., 64, 3607-3614.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | ethanol | 30% stimulation at 15%, v/v , 15% stimulation at 20%, v/v | Aspergillus oryzae |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Ag+ | nearly complete inhibition at 5 mM | Aspergillus oryzae | |
| 3.2.1.21 | beta-D-glucose | competitive inhibition, Ki: 1.36 mM | Aspergillus oryzae | |
| 3.2.1.21 | castanospermine | nearly complete inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.21 | Cu2+ | nearly complete inhibition at 5 mM | Aspergillus oryzae | |
| 3.2.1.21 | D-glucono-1,5-lactone | competitive inhibition, Ki: 12.5 mM | Aspergillus oryzae | |
| 3.2.1.21 | diethyldicarbonate | nearly complete inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.21 | Fe3+ | strong inhibition at 5 mM | Aspergillus oryzae | |
| 3.2.1.21 | Hg2+ | complete inhibition at 5 mM | Aspergillus oryzae | |
| 3.2.1.21 | methyldeoxynojirimycin | strong inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.21 | N-bromosuccinimide | complete inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.21 | SDS | strong inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.21 | Zn2+ | strong inhibition at 5 mM | Aspergillus oryzae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.55 | - |
4-nitrophenyl-beta-D-glucoside | - |
Aspergillus oryzae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | most effective induction of enzyme synthesis in medium containing quercetin | Aspergillus oryzae | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Mn2+ | 77% stimulation at 5 mM | Aspergillus oryzae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 43000 | - |
gel filtration | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Aspergillus oryzae | low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Aspergillus oryzae | sophorose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-alpha-D-glucopyranosyl-D-glucose + H2O | Aspergillus oryzae | nigerose | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Aspergillus oryzae | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-alpha-D-glucopyranosyl-D-glucose | Aspergillus oryzae | maltose | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | Aspergillus oryzae | lactose | beta-D-galactose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus oryzae | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-xylopyranosyl-D-xylose + H2O | Aspergillus oryzae | - |
beta-D-xylose + D-xylose | - |
? | |
| 3.2.1.21 | 6-O-alpha-D-glucopyranosyl-D-glucose + H2O | Aspergillus oryzae | isomaltose | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus oryzae | low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus oryzae | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | Aspergillus oryzae | - |
5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | Aspergillus oryzae | - |
4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | Aspergillus oryzae | - |
3 D-glucose | - |
? | |
| 3.2.1.21 | laminarin + H2O | Aspergillus oryzae | low activity | beta-D-glucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | to homogeneity, chromatography steps | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 1.07 | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | low activity | Aspergillus oryzae | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Aspergillus oryzae | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-alpha-D-glucopyranosyl-D-glucose + H2O | nigerose | Aspergillus oryzae | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Aspergillus oryzae | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-galactoside + H2O | low activity | Aspergillus oryzae | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-O-alpha-D-glucopyranosyl-D-glucose | maltose | Aspergillus oryzae | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Aspergillus oryzae | beta-D-galactose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus oryzae | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-xylopyranosyl-D-xylose + H2O | - |
Aspergillus oryzae | beta-D-xylose + D-xylose | - |
? | |
| 3.2.1.21 | 6-O-alpha-D-glucopyranosyl-D-glucose + H2O | isomaltose | Aspergillus oryzae | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Aspergillus oryzae | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Aspergillus oryzae | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | - |
Aspergillus oryzae | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | - |
Aspergillus oryzae | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | - |
Aspergillus oryzae | 3 D-glucose | - |
? | |
| 3.2.1.21 | laminarin + H2O | low activity | Aspergillus oryzae | beta-D-glucose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | monomer | 1 * 43000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | HGT-BG | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
- |
Aspergillus oryzae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 45 | - |
stable up to | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
- |
Aspergillus oryzae |
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