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Literature summary extracted from

  • Hill, B.; Attwood, M.M.
    Purification and characterization of phosphoglycerate mutase from methanol-grown Hyphomicrobium X and Pseudomonas AM1 (1976), J. Gen. Microbiol., 96, 185-193.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.11 2,3-diphosphoglycerate Km: 0.01 mM Pseudomonas sp.
5.4.2.11 2,3-diphosphoglycerate Km: 0.008 mM Hyphomicrobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.11 0.37
-
 2-phospho-D-glycerate
-
 Pseudomonas sp.
5.4.2.11 0.69
-
 2-phospho-D-glycerate
-
 Hyphomicrobium sp.
5.4.2.11 3.4
-
 3-phospho-D-glycerate
-
 Pseudomonas sp.
5.4.2.11 6
-
 3-phospho-D-glycerate
-
 Hyphomicrobium sp.

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.11 Hyphomicrobium sp.
-
 X
-
5.4.2.11 Hyphomicrobium sp. X
-
 X
-
5.4.2.11 Pseudomonas sp.
-
 AM1
-
5.4.2.11 Pseudomonas sp. AM1
-
 AM1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.11
-
 Pseudomonas sp.
5.4.2.11
-
 Hyphomicrobium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.11 3.22
-
-
 Hyphomicrobium sp.
5.4.2.11 20.03
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
5.4.2.11 -20°C, 10 mM sodium phosphate buffer, pH 7.0, 5 mM MgCl2, 2 mM 2-mercaptoethanol, 10% loss of activity in 1 week Pseudomonas sp.
5.4.2.11 -20°C, 10 mM sodium phosphate buffer, pH 7.0, 5 mM MgCl2, 2 mM 2-mercaptoethanol, 13% loss of activity in 1 week Hyphomicrobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.11 2-phospho-D-glycerate
-
 Pseudomonas sp. 3-phospho-D-glycerate
-
 r
5.4.2.11 2-phospho-D-glycerate
-
 Hyphomicrobium sp. 3-phospho-D-glycerate
-
 r
5.4.2.11 2-phospho-D-glycerate
-
 Pseudomonas sp. AM1 3-phospho-D-glycerate
-
 r
5.4.2.11 2-phospho-D-glycerate
-
 Hyphomicrobium sp. X 3-phospho-D-glycerate
-
 r
5.4.2.11 3-phospho-D-glycerate
-
 Pseudomonas sp. 2-phospho-D-glycerate
-
 r
5.4.2.11 3-phospho-D-glycerate
-
 Hyphomicrobium sp. 2-phospho-D-glycerate
-
 r
5.4.2.11 3-phospho-D-glycerate
-
 Pseudomonas sp. AM1 2-phospho-D-glycerate
-
 r
5.4.2.11 3-phospho-D-glycerate
-
 Hyphomicrobium sp. X 2-phospho-D-glycerate
-
 r
5.4.2.11 Hydroxypyruvic acid 3-phosphate 3-7% activity Pseudomonas sp. ?
-
 ?
5.4.2.11 Hydroxypyruvic acid 3-phosphate 3-7% activity Hyphomicrobium sp. ?
-
 ?
5.4.2.11 Hydroxypyruvic acid 3-phosphate 3-7% activity Pseudomonas sp. AM1 ?
-
 ?
5.4.2.11 Hydroxypyruvic acid 3-phosphate 3-7% activity Hyphomicrobium sp. X ?
-
 ?
5.4.2.11 additional information no substrates are beta-glycerol phosphate, DL-O-phosphoserine, DL-glyceric acid, 3-DL-chloroacetic acid, DL-Ser, DL-glyceraldehyde 3-phosphate Pseudomonas sp. ?
-
 ?
5.4.2.11 additional information no substrates are beta-glycerol phosphate, DL-O-phosphoserine, DL-glyceric acid, 3-DL-chloroacetic acid, DL-Ser, DL-glyceraldehyde 3-phosphate Hyphomicrobium sp. ?
-
 ?
5.4.2.11 additional information no substrates are beta-glycerol phosphate, DL-O-phosphoserine, DL-glyceric acid, 3-DL-chloroacetic acid, DL-Ser, DL-glyceraldehyde 3-phosphate Pseudomonas sp. AM1 ?
-
 ?
5.4.2.11 additional information no substrates are beta-glycerol phosphate, DL-O-phosphoserine, DL-glyceric acid, 3-DL-chloroacetic acid, DL-Ser, DL-glyceraldehyde 3-phosphate Hyphomicrobium sp. X ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.11 30
-
-
 Hyphomicrobium sp.
5.4.2.11 30
-
 30 min, 38% loss of activity in absence of cofactor Pseudomonas sp.
5.4.2.11 60
-
 30 min, 17% loss of activity in presence of cofactor Hyphomicrobium sp.
5.4.2.11 60
-
 30 min, 13% loss of activity in presence of cofactor Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.11 7 7.6
-
 Pseudomonas sp.
5.4.2.11 7.3
-
-
 Hyphomicrobium sp.