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Literature summary extracted from

  • Jahns, T.; Schepp, R.; Kaltwasser, H.
    Purification and characterization of an enzyme from a strain of Ochrobactrum anthropi that degrades condensation products of urea and formaldehyde (ureaform) (1997), Can. J. Microbiol., 43, 1111-1117.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.21 Acetohydroxamate
-
 Brucella anthropi
3.5.3.21 Borate
-
 Brucella anthropi
3.5.3.21 CdSO4
-
 Brucella anthropi
3.5.3.21 CuSO4
-
 Brucella anthropi
3.5.3.21 HgCl2
-
 Brucella anthropi
3.5.3.21 NiCl2
-
 Brucella anthropi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.21 38000
-
 4 * 38000, SDS-PAGE Brucella anthropi
3.5.3.21 125000
-
 native PAGE Brucella anthropi
3.5.3.21 140000
-
 gel filtration Brucella anthropi

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.21 Brucella anthropi
-
-
-
3.5.3.21 Brucella anthropi UF4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.21
-
 Brucella anthropi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.21 64.29
-
-
 Brucella anthropi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.21 Allantoate + H2O at 5% of the activity with methylenediurea Brucella anthropi Glyoxylate + urea + ammonium
-
 ?
3.5.3.21 Allantoate + H2O at 5% of the activity with methylenediurea Brucella anthropi UF4 Glyoxylate + urea + ammonium
-
 ?
3.5.3.21 dimethylenetriurea + H2O
-
 Brucella anthropi ammonium + formaldehyde + urea + CO2 ammonium:formaldehyde:urea at a molar ratio of 4:2:1 ?
3.5.3.21 dimethylenetriurea + H2O
-
 Brucella anthropi UF4 ammonium + formaldehyde + urea + CO2 ammonium:formaldehyde:urea at a molar ratio of 4:2:1 ?
3.5.3.21 methylenediurea + H2O
-
 Brucella anthropi ammonium + formaldehyde + urea + CO2 ammonium:formaldehyde:urea at a molar ratio of 2:1:1 ?
3.5.3.21 methylenediurea + H2O
-
 Brucella anthropi UF4 ammonium + formaldehyde + urea + CO2 ammonium:formaldehyde:urea at a molar ratio of 2:1:1 ?
3.5.3.21 trimethylenetetraurea + H2O
-
 Brucella anthropi ammonium + formaldehyde + urea + CO2 ammonium:formaldehyde:urea at a molar ratio of 6:3:1 ?
3.5.3.21 trimethylenetetraurea + H2O
-
 Brucella anthropi UF4 ammonium + formaldehyde + urea + CO2 ammonium:formaldehyde:urea at a molar ratio of 6:3:1 ?
3.5.3.21 Ureidoglycolate + H2O at 130% of the activity with methylenediurea Brucella anthropi Glyoxylate + urea
-
 ?
3.5.3.21 Ureidoglycolate + H2O at 130% of the activity with methylenediurea Brucella anthropi UF4 Glyoxylate + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.21 tetramer 4 * 38000, SDS-PAGE Brucella anthropi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.21 55
-
-
 Brucella anthropi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.3.21 30 70 30°C: about 45% of maximal activity, 70°C: about 30% of maximal activity Brucella anthropi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.21 70
-
 30 min, stable up to Brucella anthropi
3.5.3.21 80
-
 30 min, complete loss of activity Brucella anthropi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.21 8 8.5
-
 Brucella anthropi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.21 7 9.5 pH 7: about 25% of maximal activity, pH 9.5: about 70% of maximal activity Brucella anthropi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.21 5 11 60 min, stable Brucella anthropi