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Literature summary extracted from

  • Moriguchi, M.; Sakai, K.; Katsuno, Y.; Maki, T.; Wakayama, M.
    Purification and characterization of novel N-acyl-D-aspartate amidohydrolase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (1993), Biosci. Biotechnol. Biochem., 57, 1145-1148.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 Ca2+
-
 Alcaligenes sp.
3.5.1.14 Co2+
-
 Alcaligenes sp.
3.5.1.14 Cu2+
-
 Alcaligenes sp.
3.5.1.14 dithiothreitol
-
 Alcaligenes sp.
3.5.1.14 EDTA
-
 Alcaligenes sp.
3.5.1.14 Fe2+
-
 Alcaligenes sp.
3.5.1.14 Hg2+
-
 Alcaligenes sp.
3.5.1.14 iodoacetic acid
-
 Alcaligenes sp.
3.5.1.14 Mn2+
-
 Alcaligenes sp.
3.5.1.14 N-ethylmaleimide
-
 Alcaligenes sp.
3.5.1.14 Ni2+
-
 Alcaligenes sp.
3.5.1.14 p-chloromercuribenzoate
-
 Alcaligenes sp.
3.5.1.14 Zn2+
-
 Alcaligenes sp.
3.5.1.83 CaCl2
-
 Achromobacter xylosoxidans
3.5.1.83 CoCl2
-
 Achromobacter xylosoxidans
3.5.1.83 Cu2+
-
 Achromobacter xylosoxidans
3.5.1.83 dithiothreitol
-
 Achromobacter xylosoxidans
3.5.1.83 FeCl2
-
 Achromobacter xylosoxidans
3.5.1.83 Hg2+
-
 Achromobacter xylosoxidans
3.5.1.83 iodoacetic acid
-
 Achromobacter xylosoxidans
3.5.1.83 MnCl2
-
 Achromobacter xylosoxidans
3.5.1.83 NEM
-
 Achromobacter xylosoxidans
3.5.1.83 Ni2+
-
 Achromobacter xylosoxidans
3.5.1.83 PCMB
-
 Achromobacter xylosoxidans
3.5.1.83 ZnCl2
-
 Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 0.033
-
 N-butyryl-D-aspartate
-
 Alcaligenes sp.
3.5.1.14 0.194
-
 N-propionyl-D-aspartate
-
 Alcaligenes sp.
3.5.1.14 1.11
-
 N-glycyl-D-aspartate
-
 Alcaligenes sp.
3.5.1.14 2.52
-
 N-acetyl-D-aspartate
-
 Alcaligenes sp.
3.5.1.14 12.5
-
 N-formyl-D-aspartate
-
 Alcaligenes sp.
3.5.1.83 0.033
-
 N-Butanoyl-D-Asp
-
 Achromobacter xylosoxidans
3.5.1.83 0.194
-
 N-Propanoyl-D-Asp
-
 Achromobacter xylosoxidans
3.5.1.83 1.11
-
 N-Glycyl-D-Asp
-
 Achromobacter xylosoxidans
3.5.1.83 2.52
-
 N-Acetyl-D-Asp
-
 Achromobacter xylosoxidans
3.5.1.83 12.5
-
 N-Formyl-D-Asp
-
 Achromobacter xylosoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 54000
-
 1 * 54000, SDS-PAGE Alcaligenes sp.
3.5.1.14 56000
-
 gel filtration Alcaligenes sp.
3.5.1.83 54000
-
 1 * 54000, SDS-PAGE Achromobacter xylosoxidans
3.5.1.83 56000
-
 gel filtration Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Alcaligenes sp.
-
 L-methionine + acetate
-
 ?
3.5.1.83 additional information Achromobacter xylosoxidans inducible by N-acetyl-D-Asp ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Alcaligenes sp.
-
 xylosoxydans subsp. xylosoxydans A-6
-
3.5.1.83 Achromobacter xylosoxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14
-
 Alcaligenes sp.
3.5.1.83
-
 Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.83 175
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-D-aspartate + H2O
-
 Alcaligenes sp. ?
-
 ?
3.5.1.14 N-acetyl-DL-aspartate + H2O D-isomer, N-acetyl-D-aspartate as inducer Alcaligenes sp. acetate + DL-aspartate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Alcaligenes sp. DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Alcaligenes sp. L-methionine + acetate
-
 ?
3.5.1.14 N-butyryl-D-aspartate + H2O
-
 Alcaligenes sp. butanoate + D-aspartate
-
 ?
3.5.1.14 N-formyl-D-aspartate + H2O
-
 Alcaligenes sp. formate + D-aspartate
-
 ?
3.5.1.14 N-glycyl-D-aspartate + H2O
-
 Alcaligenes sp. glycine + D-aspartate
-
 ?
3.5.1.14 N-propionyl-D-aspartate + H2O
-
 Alcaligenes sp. proipionate + D-aspartate
-
 ?
3.5.1.83 Gly-D-Asp + H2O
-
 Achromobacter xylosoxidans Glycine + Asp
-
 ?
3.5.1.83 additional information no activity with N-carbobenzoyl-D-Asp, N-acetyl-L-Asp, N-acetyl-D-Glu Achromobacter xylosoxidans ?
-
 ?
3.5.1.83 additional information inducible by N-acetyl-D-Asp Achromobacter xylosoxidans ?
-
 ?
3.5.1.83 N-Acetyl-D-Asp + H2O
-
 Achromobacter xylosoxidans acetate + D-Asp
-
 ?
3.5.1.83 N-Butanoyl-D-Asp + H2O
-
 Achromobacter xylosoxidans Butanoate + D-Asp
-
 ?
3.5.1.83 N-Formyl-D-Asp + H2O
-
 Achromobacter xylosoxidans Formate + D-Asp
-
 ?
3.5.1.83 N-glycyl-D-Asp + H2O
-
 Achromobacter xylosoxidans Gly + D-Asp
-
 ?
3.5.1.83 N-Propanoyl-D-Asp + H2O
-
 Achromobacter xylosoxidans Propanoate + D-Asp
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 monomer 1 * 54000, SDS-PAGE Alcaligenes sp.
3.5.1.83 monomer 1 * 54000, SDS-PAGE Achromobacter xylosoxidans

Synonyms

EC Number Synonyms Comment Organism
3.5.1.14 D-AAse
-
 Alcaligenes sp.
3.5.1.14 N-Acyl-D-aspartate amidohydrolase
-
 Alcaligenes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 50
-
-
 Alcaligenes sp.
3.5.1.83 50
-
-
 Achromobacter xylosoxidans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.14 25 75
-
 Alcaligenes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.14 45
-
 thermostable up to, 60% activity remains at 50°C Alcaligenes sp.
3.5.1.83 45
-
 10 min, stable up to Achromobacter xylosoxidans
3.5.1.83 50
-
 10 min, 40% loss of activity Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 7.5 8
-
 Alcaligenes sp.
3.5.1.83 7.5 8
-
 Achromobacter xylosoxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.14 8
-
 most stable in borate buffer Alcaligenes sp.
3.5.1.83 8
-
 stable Achromobacter xylosoxidans