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Literature summary extracted from

  • Kubo, K.; Ishikura, T.; Fukagawa, Y.
    Deacetylation of PS-5, a new beta-lactam compound. III. Enzymological characterization of L-amino acid acylase and D-amino acid acylase from Pseudomonas sp. 1158 (1980), J. Antibiot., 33, 556-565.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.81 Cd2+ slightly Pseudomonas sp.
3.5.1.81 Cu2+
-
 Pseudomonas sp.
3.5.1.81 EDTA
-
 Pseudomonas sp.
3.5.1.81 Hg2+
-
 Pseudomonas sp.
3.5.1.81 KCN
-
 Pseudomonas sp.
3.5.1.81 monoiodoacetate
-
 Pseudomonas sp.
3.5.1.81 NEM
-
 Pseudomonas sp.
3.5.1.81 PCMB
-
 Pseudomonas sp.
3.5.1.81 Sn2+ slightly Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.81 Pseudomonas sp.
-
-
-
3.5.1.81 Pseudomonas sp. 1158
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.81 beta-Lactam PS-5 + H2O
-
 Pseudomonas sp. beta-Lactam NS5 + H2O
-
 ?
3.5.1.81 additional information the enzyme acts as a peptidase on dipeptides and tripeptides Pseudomonas sp. ?
-
 ?
3.5.1.81 additional information the enzyme acts as a peptidase on dipeptides and tripeptides Pseudomonas sp. 1158 ?
-
 ?
3.5.1.81 N-Acetyl-D-Ala + H2O
-
 Pseudomonas sp. acetate + D-Ala
-
 ?
3.5.1.81 N-Acetyl-D-Asn + H2O
-
 Pseudomonas sp. acetate + D-Asn
-
 ?
3.5.1.81 N-acetyl-D-Leu + H2O
-
 Pseudomonas sp. acetate + D-Leu
-
 ?
3.5.1.81 N-acetyl-D-Leu + H2O
-
 Pseudomonas sp. 1158 acetate + D-Leu
-
 ?
3.5.1.81 N-Acetyl-D-Met + H2O
-
 Pseudomonas sp. acetate + D-Met
-
 ?
3.5.1.81 N-Acetyl-D-Met + H2O
-
 Pseudomonas sp. 1158 acetate + D-Met
-
 ?
3.5.1.81 N-Acetyl-D-Phe + H2O
-
 Pseudomonas sp. acetate + D-Phe
-
 ?
3.5.1.81 N-Acetyl-D-Phe + H2O
-
 Pseudomonas sp. 1158 acetate + D-Phe
-
 ?
3.5.1.81 N-Acetyl-D-Tyr + H2O
-
 Pseudomonas sp. acetate + D-Tyr
-
 ?
3.5.1.81 N-Acetyl-Gly + H2O
-
 Pseudomonas sp. acetate + Gly
-
 ?
3.5.1.81 N-Chloroacetyl-D-Val + H2O
-
 Pseudomonas sp. Chloroacetate + D-Val
-
 ?
3.5.1.81 N-Chloroacetyl-D-Val + H2O
-
 Pseudomonas sp. 1158 Chloroacetate + D-Val
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.81 77
-
 activity increases up to 77 C Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.81 50
-
 stable below Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.81 7.4
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.81 6 9.5 pH 6.0: about 35% of maximal activity, pH 9.5: about 45% of maximal activity Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.81 6.5
-
 optimal stability Pseudomonas sp.