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Literature summary extracted from

  • Ogawa, J.; Shimizu, S.; Yamada, H.
    N-Carbamoyl-D-amino acid amidohydrolase from Comamonas sp. E222c. Purification and characterization (1993), Eur. J. Biochem., 212, 685-691.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.77 5,5'-dithiobis(2-nitrobenzoic acid)
-
Comamonas sp.
3.5.1.77 Ag+
-
Comamonas sp.
3.5.1.77 Cd2+
-
Comamonas sp.
3.5.1.77 Co2+
-
Comamonas sp.
3.5.1.77 Cu2+
-
Comamonas sp.
3.5.1.77 D-Phe 5 mM, slight product inhibition, 12% Comamonas sp.
3.5.1.77 Hg2+
-
Comamonas sp.
3.5.1.77 NEM
-
Comamonas sp.
3.5.1.77 NH4+ 100 mM, 20% inhibition Comamonas sp.
3.5.1.77 Ni2+
-
Comamonas sp.
3.5.1.77 PCMB
-
Comamonas sp.
3.5.1.77 Zn2+
-
Comamonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.77 1
-
N-carbamoyl-D-Val
-
Comamonas sp.
3.5.1.77 3.6
-
N-carbamoyl-D-Leu
-
Comamonas sp.
3.5.1.77 4.8
-
N-Carbamoyl-DL-norleucine
-
Comamonas sp.
3.5.1.77 7.5
-
N-Carbamoyl-DL-Met
-
Comamonas sp.
3.5.1.77 11.6
-
N-carbamoyl-D-Ala
-
Comamonas sp.
3.5.1.77 13.1
-
N-carbamoyl-D-p-hydroxyphenylglycine
-
Comamonas sp.
3.5.1.77 19.7
-
N-carbamoyl-D-Phe
-
Comamonas sp.
3.5.1.77 23.7
-
N-carbamoyl-D-Ser
-
Comamonas sp.
3.5.1.77 26.9
-
N-Carbamoyl-D-phenylglycine
-
Comamonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.77 Al3+ slightly enhances activity to about 110% of the standard activity Comamonas sp.
3.5.1.77 Fe2+ slightly enhances activity to about 110% of the standard activity Comamonas sp.
3.5.1.77 Fe3+ slightly enhances activity to about 110% of the standard activity Comamonas sp.
3.5.1.77 Pb2+ slightly enhances activity to about 110% of the standard activity Comamonas sp.
3.5.1.77 Sn2+ slightly enhances activity to about 110% of the standard activity Comamonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.77 40000
-
x * 40000, SDS-PAGE Comamonas sp.
3.5.1.77 40564
-
x * 40564, calculation from amino acid content Comamonas sp.
3.5.1.77 117000
-
gel filtration Comamonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.77 Comamonas sp.
-
-
-
3.5.1.77 Comamonas sp. E222c
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.77
-
Comamonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.77 0.34
-
-
Comamonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.77 N-carbamoyl-D-Ala + H2O 23% of the activity with N-carbamoyl-D-Phe Comamonas sp. D-Ala + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Ala + H2O 23% of the activity with N-carbamoyl-D-Phe Comamonas sp. E222c D-Ala + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Leu + H2O 28% of the activity with N-carbamoyl-D-Phe Comamonas sp. D-Leu + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Leu + H2O 28% of the activity with N-carbamoyl-D-Phe Comamonas sp. E222c D-Leu + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-p-hydroxyphenylglycine + H2O 47% of the activity with N-carbamoyl-D-Phe Comamonas sp. D-p-hydroxyphenylglycine + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Phe + H2O
-
Comamonas sp. D-Phe + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Phe + H2O
-
Comamonas sp. E222c D-Phe + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-phenylglycine + H2O 24% of the activity with N-carbamoyl-D-Phe Comamonas sp. D-phenylglycine + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Ser + H2O 7% of the activity with N-carbamoyl-D-Phe Comamonas sp. D-Ser + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Ser + H2O 7% of the activity with N-carbamoyl-D-Phe Comamonas sp. E222c D-Ser + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Trp + H2O 55% of the activity with N-carbamoyl-D-Phe Comamonas sp. D-Trp + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Val + H2O 10% of the activity with N-carbamoyl-D-Phe Comamonas sp. D-Val + NH3 + CO2
-
?
3.5.1.77 N-carbamoyl-D-Val + H2O 10% of the activity with N-carbamoyl-D-Phe Comamonas sp. E222c D-Val + NH3 + CO2
-
?
3.5.1.77 N-Carbamoyl-DL-2-amino-n-butyric acid + H2O 14% of the activity with N-carbamoyl-D-Phe Comamonas sp. ?
-
?
3.5.1.77 N-Carbamoyl-DL-Met + H2O 92% of the activity with N-carbamoyl-D-Phe Comamonas sp. ?
-
?
3.5.1.77 N-Carbamoyl-DL-norleucine + H2O 92% of the activity with N-carbamoyl-D-Phe Comamonas sp. ?
-
?
3.5.1.77 N-Carbamoyl-DL-norleucine + H2O 90% of the activity with N-carbamoyl-D-Phe Comamonas sp. ?
-
?
3.5.1.77 N-Carbamoyl-DL-norvaline + H2O 6% of the activity with N-carbamoyl-D-Phe Comamonas sp. ?
-
?
3.5.1.77 N-Carbamoyl-DL-Thr + H2O 3% of the activity with N-carbamoyl-D-Phe Comamonas sp. ?
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.77 ? x * 40000, SDS-PAGE Comamonas sp.
3.5.1.77 ? x * 40564, calculation from amino acid content Comamonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.77 40
-
-
Comamonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.77 30 50 30°C: about 50% of maximal activity, 50°C: about 55% of maximal activity Comamonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.77 40
-
pH 7.0, 20 min, stable below Comamonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.77 8 9 N-carbamoyl-D-Phe Comamonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.77 5 11 pH 5.0: about 40% of maximal activity, pH 11: about 25% of maximal activity Comamonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.77 7 9 30°C, 20 min, stable Comamonas sp.