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Literature summary extracted from
- A protease in the venom of king cobra (Ophiophagus hannah): purification, characterization and substrate specificity on oxidized insulin B-chain (1988), Toxicon, 26, 1145-1155.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.51 | EDTA | - |
Ophiophagus hannah |  |
| 3.4.24.51 | formaldehyde | - |
Ophiophagus hannah | |
| 3.4.24.51 | Mn2+ | - |
Ophiophagus hannah | |
| 3.4.24.51 | additional information | phenylmethylsulfonyl fluoride or N-ethylmaleimide are no inhibitors | Ophiophagus hannah | |
| 3.4.24.51 | PMSF | - |
Ophiophagus hannah | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.51 | additional information | Mg2+ has no influence | Ophiophagus hannah |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.51 | 65000 | - |
Ophiophagus hannah, gel filtration | Ophiophagus hannah |
| 3.4.24.51 | 70000 | - |
1 * 70000, Ophiophagus hannah, SDS-PAGE | Ophiophagus hannah |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.51 | Ophiophagus hannah | - |
king cobra | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.51 | partial | Ophiophagus hannah |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.51 | venom | - |
Ophiophagus hannah | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.51 | Insulin B-chain + H2O | cleavage of Asn3-Gln, Gln4-His, His10-Leu, Ala14-Leu and Tyr16-Leu | Ophiophagus hannah | Hydrolyzed insulin B-chain | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.51 | monomer | 1 * 70000, Ophiophagus hannah, SDS-PAGE | Ophiophagus hannah |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.51 | 37 | - |
assay at | Ophiophagus hannah |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.51 | 7.2 | - |
assay at | Ophiophagus hannah |
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