EC Number | General Stability | Organism |
---|---|---|
3.4.24.49 | Ca2+ stabilizes | Bothrops jararaca |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.49 | EDTA | complete inhibition | Bothrops jararaca | |
3.4.24.49 | EGTA | complete inhibition | Bothrops jararaca | |
3.4.24.49 | oxalate | 85% inhibition, restorage of 85% of activity by removal of oxalate by addition of Ca2+ | Bothrops jararaca |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.49 | Ca2+ | 1 mM required for maximal activity | Bothrops jararaca |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.49 | 37300 | - |
Bothrops jararaca, sedimentation equilibrium centrifugation in presence of EDTA | Bothrops jararaca |
3.4.24.49 | 48000 | - |
x * 37300, Bothrops jararaca, SDS-PAGE in presence of EDTA, x * 48000, Bothrops jararaca, SDS-PAGE | Bothrops jararaca |
3.4.24.49 | 49870 | - |
Bothrops jararaca, sedimentation equilibrium centrifugation in presence of 6 M guanidine-HCl and 0.1 M 2-mercaptoethanol | Bothrops jararaca |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.49 | Bothrops jararaca | - |
jararaca snake | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.49 | partial | Bothrops jararaca |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.49 | venom | - |
Bothrops jararaca | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.49 | casein + H2O | endopeptidase | Bothrops jararaca | hydrolyzed casein | - |
? | |
3.4.24.49 | Insulin B-chain + H2O | cleavage of His5-Leu, His10-Leu, Ala14-Leu, Tyr16-Leu and Phe24-Phe | Bothrops jararaca | Hydrolyzed insulin B-chain | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.49 | monomer | x * 37300, Bothrops jararaca, SDS-PAGE in presence of EDTA, x * 48000, Bothrops jararaca, SDS-PAGE | Bothrops jararaca |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.49 | 20 | - |
assay at | Bothrops jararaca |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.49 | 7 | - |
assay at | Bothrops jararaca |