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Literature summary extracted from

  • Kim, N.; Kim, S.I.
    Metal ion relevance for the stability and activity of a 52-kDa Serratia proteinase (1993), Biosci. Biotechnol. Biochem., 57, 29-33.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 EDTA Ca2+ (restored activity higher than native activity); enhances inhibition by guanidine-HCl; Fe2+ (partially); Mg2+; Mn2+ (restored activity higher than native activity); no reactivation by K+; Zn2+ (restored activity higher than native activity) Serratia marcescens
3.4.24.40 Guanidine-HCl enhanced by EDTA Serratia marcescens
3.4.24.40 additional information PCMB Serratia marcescens
3.4.24.40 PMSF not Serratia marcescens
3.4.24.40 Tetraethylenepentamine zinc-specific chelator Serratia marcescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.40 extracellular
-
 Serratia marcescens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Zn2+ zinc-containing metalloproteinase Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 52000
-
 1 * 52000, Serratia marcescens, SDS-PAGE Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Serratia marcescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40
-
 Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 Azocoll + H2O
-
 Serratia marcescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.40 monomer 1 * 52000, Serratia marcescens, SDS-PAGE Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.40 additional information
-
 thermal unfolding profiles Serratia marcescens
3.4.24.40 55
-
 the selective substitution of Ca2+ for functional zinc does not change stability below 55°¦C Serratia marcescens
3.4.24.40 60
-
 inactivation in 20 mM Tris-HCl buffer, pH 7, stable in the presence of tetraethylenepentamine or CaCl2 Serratia marcescens