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Literature summary extracted from

  • Maeda, H.; Morihara, K.
    Serralysin and related bacterial proteinases (1995), Methods Enzymol., 248, 395-413.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.40 Pseudomonas aeruginosa Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 1,10-phenanthroline
-
 Citrobacter freundii
3.4.24.40 1,10-phenanthroline
-
 Pseudomonas aeruginosa
3.4.24.40 1,10-phenanthroline Serratia marcescens enzyme Serratia marcescens
3.4.24.40 antipain only Serratia marcescens MW 56000 enzyme Serratia marcescens
3.4.24.40 Cysteine hydrochloride not Serratia marcescens enzyme Citrobacter freundii
3.4.24.40 Cysteine hydrochloride not Serratia marcescens enzyme Dickeya chrysanthemi
3.4.24.40 Cysteine hydrochloride not Serratia marcescens enzyme Proteus mirabilis
3.4.24.40 Cysteine hydrochloride not Serratia marcescens enzyme Pseudomonas aeruginosa
3.4.24.40 Cysteine hydrochloride not Serratia marcescens enzyme Pseudomonas fragi
3.4.24.40 Cysteine hydrochloride not Serratia marcescens enzyme Serratia marcescens
3.4.24.40 Cysteine hydrochloride not Serratia marcescens enzyme Serratia sp. E-15
3.4.24.40 DTT weak, Serratia marcescens Citrobacter freundii
3.4.24.40 DTT weak, Serratia marcescens Dickeya chrysanthemi
3.4.24.40 DTT weak, Serratia marcescens Proteus mirabilis
3.4.24.40 DTT weak, Serratia marcescens Pseudomonas aeruginosa
3.4.24.40 DTT weak, Serratia marcescens Pseudomonas fragi
3.4.24.40 DTT weak, Serratia marcescens Serratia marcescens
3.4.24.40 DTT weak, Serratia marcescens Serratia sp. E-15
3.4.24.40 EDTA Mn2+ Citrobacter freundii
3.4.24.40 EDTA
-
 Dickeya chrysanthemi
3.4.24.40 EDTA Mn2+ Pseudomonas aeruginosa
3.4.24.40 EDTA Ca2+ (MW 56000 and MW 60000 enzyme); Ca2+ (partially); Co2+ (Serratia sp. E-16 enzyme); Cu2+ (not); Fe2+ (only MW 56000 enzyme); Mg2+ (not); Serratia enzymes; Zn2+ (MW 56000 and MW 60000 enzyme) Serratia marcescens
3.4.24.40 leupeptin weak, only Serratia marcescens MW 56000 enzyme Serratia marcescens
3.4.24.40 additional information
-
 Citrobacter freundii
3.4.24.40 additional information
-
 Pseudomonas aeruginosa
3.4.24.40 additional information 2-mercaptoethanol (Serratia marcescens); diisopropyl phosphofluoridate (Serratia marcescens); GSH, EGTA (Serratia marcescens), phosphoramidon, zincov (i.e. 2-(N-hydroxycarboxamido)-4-methylpentanoyl-L-Ala-glycinamide, thermolysin inhibitor); monoiodoacetic acid (Serratia marcescens) Serratia marcescens
3.4.24.40 N-ethylmaleimide not (Serratia marcescens enzyme) Citrobacter freundii
3.4.24.40 N-ethylmaleimide not (Serratia marcescens enzyme) Dickeya chrysanthemi
3.4.24.40 N-ethylmaleimide not (Serratia marcescens enzyme) Proteus mirabilis
3.4.24.40 N-ethylmaleimide not (Serratia marcescens enzyme) Pseudomonas aeruginosa
3.4.24.40 N-ethylmaleimide not (Serratia marcescens enzyme) Pseudomonas fragi
3.4.24.40 N-ethylmaleimide not (Serratia marcescens enzyme) Serratia marcescens
3.4.24.40 N-ethylmaleimide not (Serratia marcescens enzyme) Serratia sp. E-15
3.4.24.40 PMSF weak, Serratia marcescens Serratia marcescens
3.4.24.40 Tetramethylenepentamine Serratia marcescens Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.40 0.4
-
 Benzyloxycarbonyl-Phe-Ala-Ala
-
 Pseudomonas aeruginosa
3.4.24.40 0.6
-
 benzyloxycarbonyl-Gly-Leu-Phe benzyloxycarbonyl-Ala-Phe-Leu-Ala Pseudomonas aeruginosa
3.4.24.40 2.5
-
 benzyloxycarbonyl-Val-Ala-Ala benzyloxycarbonyl-Ala-Phe-Gly-Ala Pseudomonas aeruginosa
3.4.24.40 2.7
-
 Benzyloxycarbonyl-Ala-Ala-Ala
-
 Pseudomonas aeruginosa
3.4.24.40 4.3
-
 Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Ala
-
 Pseudomonas aeruginosa
3.4.24.40 5.3
-
 Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala
-
 Pseudomonas aeruginosa
3.4.24.40 5.4
-
 benzyloxycarbonyl-Gly-Gly-Leu-Ala benzyloxycarbonyl-Ala-Gly-Gly-Leu Pseudomonas aeruginosa
3.4.24.40 7.7
-
 Benzyloxycarbonyl-Gly-Ala-Ala
-
 Pseudomonas aeruginosa
3.4.24.40 11
-
 Benzyloxycarbonyl-Gly-Leu-Gly-Gly
-
 Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.40 extracellular
-
 Pseudomonas aeruginosa
-
-
3.4.24.40 extracellular
-
 Serratia marcescens
-
-
3.4.24.40 extracellular
-
 Proteus mirabilis
-
-
3.4.24.40 extracellular
-
 Pseudomonas fragi
-
-
3.4.24.40 extracellular
-
 Dickeya chrysanthemi
-
-
3.4.24.40 extracellular
-
 Citrobacter freundii
-
-
3.4.24.40 extracellular
-
 Serratia sp. E-15
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Ca2+
-
 Pseudomonas aeruginosa
3.4.24.40 Ca2+
-
 Dickeya chrysanthemi
3.4.24.40 Ca2+
-
 Citrobacter freundii
3.4.24.40 Ca2+ weakly restores activity after EDTA treatment, Serratia marcescens MW 56000 and MW 60000 enzymes Serratia marcescens
3.4.24.40 Co2+ activation Pseudomonas aeruginosa
3.4.24.40 Co2+ activation Serratia marcescens
3.4.24.40 Co2+ activation Citrobacter freundii
3.4.24.40 Fe2+ restores activity after EDTA treatment, Serratia marcescens MW 56000 enzyme, not MW 60000 enzyme Serratia marcescens
3.4.24.40 Zn2+ restores activity after EDTA treatment (Serratia marcescens MW 56000 and MW 60000 enzymes) Serratia marcescens
3.4.24.40 Zn2+ 1 gatom/mol enzyme (Serratia marcescens, Serratia sp., Serratia piscatrum) Serratia marcescens
3.4.24.40 Zn2+ 1 gatom/mol enzyme (Serratia marcescens, Serratia sp., Serratia piscatrum) Serratia sp. E-15
3.4.24.40 Zn2+ 1 mol/mol enzyme Serratia marcescens
3.4.24.40 Zn2+ 1 mol/mol enzyme Serratia sp. E-15
3.4.24.40 Zn2+ zinc-binding motif: His-Glu-Xaa-Xaa-His Pseudomonas aeruginosa
3.4.24.40 Zn2+ zinc-binding motif: His-Glu-Xaa-Xaa-His Serratia marcescens
3.4.24.40 Zn2+ zinc-binding motif: His-Glu-Xaa-Xaa-His Dickeya chrysanthemi
3.4.24.40 Zn2+ zinc-binding motif: His-Glu-Xaa-Xaa-His Serratia sp. E-15
3.4.24.40 Zn2+ zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15) Pseudomonas aeruginosa
3.4.24.40 Zn2+ zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15) Dickeya chrysanthemi
3.4.24.40 Zn2+ zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15) Serratia sp. E-15
3.4.24.40 Zn2+ zinc-containing metalloproteinase Pseudomonas aeruginosa
3.4.24.40 Zn2+ zinc-containing metalloproteinase Serratia marcescens
3.4.24.40 Zn2+ zinc-containing metalloproteinase Dickeya chrysanthemi
3.4.24.40 Zn2+ zinc-containing metalloproteinase Citrobacter freundii
3.4.24.40 Zn2+ zinc-containing metalloproteinase Serratia sp. E-15

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 additional information
-
 primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme Pseudomonas aeruginosa
3.4.24.40 additional information
-
 primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme Serratia marcescens
3.4.24.40 additional information
-
 primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme Dickeya chrysanthemi
3.4.24.40 additional information
-
 primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme Citrobacter freundii
3.4.24.40 additional information
-
 primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme Serratia sp. E-15
3.4.24.40 49500
-
 Pseudomonas aeruginosa, deduced from nucleotide sequence Pseudomonas aeruginosa
3.4.24.40 50600
-
 Serratia piscatrum, deduced from nucleotide sequence Serratia sp. E-15
3.4.24.40 56000
-
 Serratia marcescens, strain kums, two enzymes: MW 56000 and MW 60000 Serratia marcescens
3.4.24.40 60000
-
 Serratia marcescens, two enzymes: MW 56000 and MW 60000 Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Citrobacter freundii
-
-
-
3.4.24.40 Dickeya chrysanthemi
-
 plant pathogen
-
3.4.24.40 Proteus mirabilis
-
-
-
3.4.24.40 Pseudomonas aeruginosa
-
-
-
3.4.24.40 Pseudomonas fragi
-
-
-
3.4.24.40 Serratia marcescens
-
 strain kums 3958 (clinical isolate)
-
3.4.24.40 Serratia sp. E-15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40
-
 Pseudomonas aeruginosa
3.4.24.40 2 immunologically not related enzymes: MW 56000 and MW 60000 Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.40 additional information
-
-
 Pseudomonas aeruginosa
3.4.24.40 additional information
-
-
 Serratia marcescens

Storage Stability

EC Number Storage Stability Organism
3.4.24.40 -70°C, lyophilized Serratia marcescens enzyme, several months Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 2-Aminobenzoyl-Gly-Phe-Arg-Leu-Leu 4-nitrobenzyl amide + H2O Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 2-Aminobenzoyl-Gly-Phe-Arg-Leu-Leu 4-nitrobenzyl amide + H2O Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme Serratia marcescens ?
-
 ?
3.4.24.40 2-Aminobenzoyl-Gly-Phe-Arg-Xaa 4-nitrobenzyl amide + H2O Xaa is Gly, Ala, Val, Leu or Phe, Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 2-Aminobenzoyl-Gly-Phe-Arg-Xaa 4-nitrobenzyl amide + H2O Xaa is Gly, Ala, Val, Leu or Phe, Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme Serratia marcescens ?
-
 ?
3.4.24.40 Azocoll + H2O
-
 Serratia marcescens ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Ala-Ala-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Ala-Gly-Gly-Leu + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Ala-Gly-Gly-Leu-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Ala-Phe-Gly-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Ala-Phe-Leu-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Gly-Ala-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 benzyloxycarbonyl-Gly-Gly-Leu-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Gly-Gly + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Phe + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Phe-Ala-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme Pseudomonas aeruginosa Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.24.40 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme Serratia marcescens Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.24.40 benzyloxycarbonyl-Val-Ala-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 casein + H2O
-
 Serratia marcescens ?
-
 ?
3.4.24.40 casein + H2O
-
 Proteus mirabilis ?
-
 ?
3.4.24.40 casein + H2O
-
 Pseudomonas fragi ?
-
 ?
3.4.24.40 casein + H2O
-
 Dickeya chrysanthemi ?
-
 ?
3.4.24.40 casein + H2O
-
 Citrobacter freundii ?
-
 ?
3.4.24.40 casein + H2O
-
 Serratia sp. E-15 ?
-
 ?
3.4.24.40 Gelatin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Gelatin + H2O
-
 Dickeya chrysanthemi ?
-
 ?
3.4.24.40 Immunoglobulin A1 + H2O Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region Serratia marcescens ?
-
 ?
3.4.24.40 Immunoglobulin A2 + H2O Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region Serratia marcescens ?
-
 ?
3.4.24.40 Immunoglobulin G1 + H2O Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region Serratia marcescens ?
-
 ?
3.4.24.40 Immunoglobulin G2 + H2O Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region Serratia marcescens ?
-
 ?
3.4.24.40 Immunoglobulin G3 + H2O Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region Serratia marcescens ?
-
 ?
3.4.24.40 Immunoglobulin G4 + H2O Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region Serratia marcescens ?
-
 ?
3.4.24.40 additional information
-
 Serratia marcescens ?
-
 ?
3.4.24.40 additional information elastin Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 additional information broad substrate side-chain specificity: hydrophobic amino acid residues at P2 and P2' are favorable Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Oxidized insulin B-chain + H2O cleavage specificity of Pseudomonas aeruginosa, Pseudomonas fragi, Serratia sp. and Proteus mirabilis enzyme Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Oxidized insulin B-chain + H2O cleavage specificity of Pseudomonas aeruginosa, Pseudomonas fragi, Serratia sp. and Proteus mirabilis enzyme Proteus mirabilis ?
-
 ?
3.4.24.40 Oxidized insulin B-chain + H2O cleavage specificity of Pseudomonas aeruginosa, Pseudomonas fragi, Serratia sp. and Proteus mirabilis enzyme Pseudomonas fragi ?
-
 ?
3.4.24.40 Oxidized insulin B-chain + H2O cleavage specificity of Pseudomonas aeruginosa, Pseudomonas fragi, Serratia sp. and Proteus mirabilis enzyme Serratia sp. E-15 ?
-
 ?
3.4.24.40 Plasma fibronectin + H2O Serratia marcescens MW 52000 enzyme, cleavage sites Serratia marcescens ?
-
 ?
3.4.24.40 Plasma fibronectin + H2O from pig Serratia marcescens ?
-
 ?
3.4.24.40 Pro-Phe-Arg 4-methylcoumarin 7-amide + H2O Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme Pseudomonas aeruginosa Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.24.40 Pro-Phe-Arg 4-methylcoumarin 7-amide + H2O Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme Serratia marcescens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.40 More belongs to serralysin subfamily M12 Pseudomonas aeruginosa
3.4.24.40 More belongs to serralysin subfamily M12 Serratia marcescens
3.4.24.40 More belongs to serralysin subfamily M12 Proteus mirabilis
3.4.24.40 More belongs to serralysin subfamily M12 Pseudomonas fragi
3.4.24.40 More belongs to serralysin subfamily M12 Dickeya chrysanthemi
3.4.24.40 More belongs to serralysin subfamily M12 Citrobacter freundii
3.4.24.40 More belongs to serralysin subfamily M12 Serratia sp. E-15

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 6 10 broad Pseudomonas aeruginosa
3.4.24.40 6 10 broad Serratia marcescens