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Literature summary extracted from

  • Matsuda, Y.; Saito, T.; Yamaguchi, T.; Kawase, H.
    Cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii is a metalloprotease and digests the sodium perchlorate-insoluble component of cell wall (1985), J. Biol. Chem., 260, 6373-6377.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.38 aspartate
-
 Chlamydomonas reinhardtii
3.4.24.38 CDTA
-
 Chlamydomonas reinhardtii
3.4.24.38 Co2+ above 1-2 mM, native or apoenzyme Chlamydomonas reinhardtii
3.4.24.38 Cu(CH3COO)2
-
 Chlamydomonas reinhardtii
3.4.24.38 cysteine
-
 Chlamydomonas reinhardtii
3.4.24.38 EDTA inactivation by dialysis, 1-2 mM Zn2+, Mn2+ or Co2+ restores activity of metall-free apoenzyme to 0.5%, 10% and 50% of native enzyme, respectively, no restoring by Mg2+, Ca2+, Cu2+ or Fe3+; most effectively reversed by Co2+ Chlamydomonas reinhardtii
3.4.24.38 EGTA about 25% as effective as EDTA or CDTA Chlamydomonas reinhardtii
3.4.24.38 glutamate
-
 Chlamydomonas reinhardtii
3.4.24.38 histidine
-
 Chlamydomonas reinhardtii
3.4.24.38 Mn2+ above 1-2 mM, native or apoenzyme Chlamydomonas reinhardtii
3.4.24.38 additional information chymostatin, antipain, leupeptin; pepstatin A; various amino acids, protease inhibitor E-64 Chlamydomonas reinhardtii
3.4.24.38 phosphoramidon
-
 Chlamydomonas reinhardtii
3.4.24.38 Zn2+ above 1-2 mM, native or apoenzyme Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.38 additional information the enzyme does not contain cobalt or manganese Chlamydomonas reinhardtii
3.4.24.38 Zinc zinc enzyme, 2.5 gatom zinc/62000 MW enzyme subunit, atomic absorption spectroscopy Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.38 60000
-
 x * 60000, Chlamydomonas reinhardtii, SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.38 Cell wall protein + H2O Chlamydomonas reinhardtii released during agglutination of gametes of opposite mating type ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.38 Chlamydomonas reinhardtii
-
 green alga, wild-type strain 137c
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.38 additional information isolated from gametes Chlamydomonas reinhardtii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.38 azocasein + H2O poor substrate Chlamydomonas reinhardtii fragments of azocasein
-
 ?
3.4.24.38 casein + H2O alpha- and beta-casein, poor substrate Chlamydomonas reinhardtii polypeptides derived from casein
-
 ?
3.4.24.38 Cell wall protein + H2O released during agglutination of gametes of opposite mating type Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 Chlamydomonas cell wall polypeptide + H2O 2 polypeptides (peptide 2, MW 294000, and 5, MW 215000) of 16 polypeptides, i.e. the NaClO4-insoluble fraction, disintegrates cell wall structure by acting on only a few salt-insoluble components Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 additional information bovine serum albumin Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 additional information no substrates are cytochrome c, lysozyme, myoglobin, trypsin inhibitor, ovalbumin Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 RNAse A + H2O
-
 Chlamydomonas reinhardtii Lower MW polypetides
-
 ?
3.4.24.38 Zoosporangia cell walls + H2O glutaraldehyde-fixed Chlamydomonas reinhardtii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.38 ? x * 60000, Chlamydomonas reinhardtii, SDS-PAGE Chlamydomonas reinhardtii