EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.30 | 3,4,5,6-Tetrachloro-1,2-benzoquinone | i.e. o-chloranil, 0.003 mM, most potent inactivator | Enterococcus faecalis | |
3.4.24.30 | diethylpyrocarbonate | partially reversed by hydroxylamine (not) | Enterococcus faecalis | |
3.4.24.30 | ethanol | - |
Enterococcus faecalis | |
3.4.24.30 | High MW factor of rat inflammatory exudate | strong, MW: 720000-750000 | Enterococcus faecalis | |
3.4.24.30 | L-Leucine hydroxamate | - |
Enterococcus faecalis | |
3.4.24.30 | methanol | about 15% as effective as n-butanol | Enterococcus faecalis | |
3.4.24.30 | additional information | no inhibition by 2,3-butanedinone; no inhibition by PMSF, phenylglyoxal | Enterococcus faecalis | |
3.4.24.30 | n-butanol | - |
Enterococcus faecalis | |
3.4.24.30 | N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline | 5 mM | Enterococcus faecalis | |
3.4.24.30 | phosphoramidon | i.e. N-(alpha-L-rhamnopyranosyloxy-hydroxyphosphinyl)-L-Leu-L-Trp | Enterococcus faecalis | |
3.4.24.30 | Propanol | - |
Enterococcus faecalis | |
3.4.24.30 | Tetranitromethane | 5 mM, pH-dependent | Enterococcus faecalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.30 | 0.02 | - |
Substance P | scissile bond Phe7-Phe8 | Enterococcus faecalis | |
3.4.24.30 | 0.049 | - |
Substance P | scissile bond Gln6-Phe7 | Enterococcus faecalis | |
3.4.24.30 | 0.0725 | - |
Insulin B-chain | scissile bond Phe24-Phe25 | Enterococcus faecalis | |
3.4.24.30 | 0.12 | - |
Substance P | scissile bond Gly9-Leu10 | Enterococcus faecalis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.30 | extracellular | - |
Enterococcus faecalis | - |
- |
3.4.24.30 | soluble | - |
Enterococcus faecalis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.30 | Zinc | requirement, metalloendopeptidase | Enterococcus faecalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.30 | additional information | - |
amino acid composition shows similarity to Staphylococcus aureus metalloendopeptidase | Enterococcus faecalis |
3.4.24.30 | 29500 | - |
Streptococcus faecalis, FPLC gel filtration | Enterococcus faecalis |
3.4.24.30 | 32000 | - |
Streptococcus faecalis, gel filtration | Enterococcus faecalis |
3.4.24.30 | 33000 | - |
1 * 33000, Streptococcus faecalis, SDS-PAGE | Enterococcus faecalis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.30 | Enterococcus faecalis | - |
var. liquefaciens, strain OG1-10 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.30 | - |
Enterococcus faecalis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.30 | culture filtrate | - |
Enterococcus faecalis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.30 | additional information | - |
- |
Enterococcus faecalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.30 | Ala-Leu-Ile-Leu-Thr-Leu-Val-Ser + H2O | i.e. iPDI, competitive inhibitor of Streptococcus faecalis sex pheromones | Enterococcus faecalis | Ala-Leu-Ile-Leu-Thr + Leu-Val-Ser | - |
? | |
3.4.24.30 | angiotensin I + H2O | i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu | Enterococcus faecalis | Asp-Arg-Val-Tyr + Ile-His-Pro + Phe-His-Leu | - |
? | |
3.4.24.30 | angiotensin II + H2O | i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe | Enterococcus faecalis | Asp-Arg-Val-Tyr + Ile-His-Pro-Phe | - |
? | |
3.4.24.30 | Azoalbumin + H2O | bovine albumin, fraction V | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | azocasein + H2O | - |
Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Azocoll + H2O | general protease substrate | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | beta-Lipotropin | cleavage site: Gly63-Phe64, poor substrate | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Bovine casein-yellow + H2O | - |
Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Bovine fibrinogen + H2O | plasminogen-free or fraction I | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Bovine hemoglobin + H2O | - |
Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Bovine pancreas insulin + H2O | poor substrate | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | bradykinin + H2O | - |
Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Cholecystokinin + H2O | cleavage site: Gly29-Trp30 | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Collagen + H2O | high or low viscose polypetides from collagen | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Gelatin + H2O | - |
Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Glucagon + H2O | cleavage site: Trp25-Leu26 | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Insulin A-chain + H2O | cleavage at Gln15-Leu16 and Tyr14-Gln15, to a much lesser extent than B-chain | Enterococcus faecalis | Fragments of insulin A-chain | peptides (Gln1-Gln15) and (Leu16-Asn21), another product formed: peptide (Gln1-Trp14) | ? | |
3.4.24.30 | Insulin B-chain + H2O | oxidized, best substrate, preferred cleavage at Phe24-Phe25, followed by His5-Leu6, after prolonged incubation: Ala14-Leu15 and His10-Leu11 | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Leu-Phe-Ser-Leu-Val-Leu-Ala-Gly + H2O | i.e. cADI, Streptococcus faecalis sex pheromone, poor substrate | Enterococcus faecalis | Leu-Phe-Ser + Leu-Val-Leu-Ala-Gly | - |
? | |
3.4.24.30 | Leu-Phe-Val-Val-Thr-Leu-Val-Gly + H2O | i.e. iADI, competitive inhibitor of Streptococcus faecalis sex pheromones | Enterococcus faecalis | Leu-Phe-Val-Val-Thr + Leu-Val-Gly | - |
? | |
3.4.24.30 | Leucine-enkephalin + H2O | cleavage site: Gly3-Phe4, poor substrate | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | Lys-bradykinin + H2O | - |
Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | additional information | strict specificity for hydrophobic amino acid at position P1', exhibits endopeptidase and thermolysin-like specificity | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | additional information | very poor substrates are elastin-orcein or hide powder azure | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | additional information | No substrates are Ala-Ile-Phe-Ile-Ala-Ser (i.e. Streptococcus faecalis sex pheromone cAM373), aminoacyl-2-naphthylamides, Nalpha-benzoyl-DL-Arg 4-nitroanilide, phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg, 2-furylacryloyl-L-Leu-Gly-L-Pro-L-Ala | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | N-3-(2-Furyl)acryloyl-Gly-L-Leu amide + H2O | chromogenic substrate, cleavage site: Gly-Leu, poor substrate | Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | neurotensin + H2O | cleavage site: Tyr11-Ile12, poor substrate | Enterococcus faecalis | fragments of neurotensin | peptides (Arg1-Gly9) and (Leu10-Met11) are the first occuring products | ? | |
3.4.24.30 | Phe-Leu-Val-Met-Phe-Leu-Ser-Gly + H2O | i.e. cPDI, Streptococcus faecalis sex pheromone, poor substrate | Enterococcus faecalis | Phe-Leu-Val-Met-Phe + Leu-Ser-Gly | - |
? | |
3.4.24.30 | Substance P + H2O | i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 | Enterococcus faecalis | Arg-Pro-Lys-Pro-Gln-Gln + Phe + Phe-Gly + Leu-Met-NH2 | - |
? | |
3.4.24.30 | Substance P + H2O | - |
Enterococcus faecalis | ? | - |
? | |
3.4.24.30 | [Lys1]bradykinin + H2O | - |
Enterococcus faecalis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.30 | monomer | 1 * 33000, Streptococcus faecalis, SDS-PAGE | Enterococcus faecalis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.30 | 37 | - |
assay at | Enterococcus faecalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.30 | additional information | - |
pI: 4.6 | Enterococcus faecalis |
3.4.24.30 | 6 | 8 | broad, azocoll as substrate | Enterococcus faecalis |
3.4.24.30 | 7.2 | - |
cleavage of Ser3-Leu4 in pheromone cAD1 | Enterococcus faecalis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.30 | 5.6 | - |
above, 17 h stable in 50 mM 2-[N-morpholino]ethanesulfonic acid buffer | Enterococcus faecalis |