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Literature summary extracted from

  • Morihara, K.; Tsuzuki, H.; Oka, T.
    Comparison of the specificities of various neutral proteinases from microorganisms (1968), Arch. Biochem. Biophys., 123, 572-588.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.27 EDTA
-
 Aspergillus oryzae
3.4.24.27 EDTA
-
 Bacillus thermoproteolyticus
3.4.24.28 EDTA
-
 Bacillus subtilis
3.4.24.31 EDTA
-
 Streptomyces griseus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Aspergillus oryzae
-
-
-
3.4.24.27 Bacillus thermoproteolyticus
-
-
-
3.4.24.28 Bacillus subtilis
-
-
-
3.4.24.31 Streptomyces griseus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 additional information specificity overview: various synthetic peptides Aspergillus oryzae ?
-
 ?
3.4.24.27 additional information specificity overview: various synthetic peptides Bacillus thermoproteolyticus ?
-
 ?
3.4.24.27 Oxidized insulin B-chain + H2O major cleavage at the peptide bonds of His5-Leu6, His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Leu17-Val18, Gly23-Phe24, Phe24-Phe25, Phe25-Tyr26 Aspergillus oryzae ?
-
 ?
3.4.24.28 additional information specificity overview: various synthetic peptides Bacillus subtilis ?
-
 ?
3.4.24.28 Oxidized insulin B-chain + H2O major cleavages at the peptide bonds of His5-Leu6, His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Gly23-Phe24 and Phe24-Phe25 Bacillus subtilis ?
-
 ?
3.4.24.31 additional information specificity overview: synthetic peptides Streptomyces griseus ?
-
 ?
3.4.24.31 Oxidized insulin B-chain + H2O cleavage at: Phe1-Val2, His5-Leu6, His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Leu17-Val18, Gly23-Phe24, Phe24-Phe25, Phe25-Tyr26 Streptomyces griseus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.28 7
-
-
 Bacillus subtilis
3.4.24.31 7 8.5
-
 Streptomyces griseus