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Literature summary extracted from

  • Sires, U.I.; Griffin, G.L.; Broekelmann, T.J.; Mecham, R.P.; Murphy, G.; Chung, A.E.; Welgus, H.G.; Senior, R.M.
    Degradation of entactin by matrix metalloproteinases. Susceptibility to matrilysin and identification of cleavage sites (1993), J. Biol. Chem., 268, 2069-2074.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.23 0.00089
-
 entactin
-
 Mammalia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.23 additional information Mammalia potentially important role of the enzyme in disruption of basement membranes by tumor or inflammatory cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.23 Mammalia
-
 enzyme is obtained by transfection of linearized PEE12 PUMP expression vector into NSO mouse myeloma cells
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.23 entactin + H2O basement membrane protein which bridges laminin and type IV collagen, enzyme produces multiple but distict cleavages in entactin resulting in peptide fragments ranging from 29000 to 115000 MW, cleavage sites: Glu34-Leu35, Gly275-Leu276, Ala637-Leu638, Cys747-Ile748 Mammalia peptide fragment ranging from 29000 to 115000 MW
-
 ?
3.4.24.23 additional information potentially important role of the enzyme in disruption of basement membranes by tumor or inflammatory cells Mammalia ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.23 37
-
 assay at Mammalia