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Literature summary extracted from
- Invertebrate proteases (1981), Methods Enzymol., 80C, 633-664.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.21 | additional information | polyvalent bovine pancreas inhibitor, porcine pancreas inhibitor, soybean trypsin inhibitor, lima bean trypsin inhibitor, ovomucoid, alpha1-antitrypsin, hirudin, pepstatin | Astacus astacus | |
| 3.4.24.21 | additional information | polyvalent bovine pancreas inhibitor, porcine pancreas inhibitor, soybean trypsin inhibitor, lima bean trypsin inhibitor, ovomucoid, alpha1-antitrypsin, hirudin, pepstatin | Astacus leptodactylus | |
| 3.4.24.21 | additional information | polyvalent bovine pancreas inhibitor, porcine pancreas inhibitor, soybean trypsin inhibitor, lima bean trypsin inhibitor, ovomucoid, alpha1-antitrypsin, hirudin, pepstatin | Carcinus maenas |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.21 | soluble | - |
Astacus astacus | - |
- |
| 3.4.24.21 | soluble | - |
Astacus leptodactylus | - |
- |
| 3.4.24.21 | soluble | - |
Carcinus maenas | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.21 | Astacus astacus | - |
i.e. Astacus fluviatilis | - |
| 3.4.24.21 | Astacus leptodactylus | - |
crayfish | - |
| 3.4.24.21 | Carcinus maenas | - |
crab | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.21 | two closely related forms of slightly different electrophoretic mobility | Astacus astacus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.21 | cardia | - |
Astacus astacus | - |
| 3.4.24.21 | cardia | synthesized in the hepatopancreas (midgut gland) and stored extracellularly in the cardia | Astacus leptodactylus | - |
| 3.4.24.21 | cardia | synthesized in the hepatopancreas (midgut gland) and stored extracellularly in the cardia | Carcinus maenas | - |
| 3.4.24.21 | digestive juice | 1 mg astacin/ml | Astacus astacus | - |
| 3.4.24.21 | digestive juice | 1 mg astacin/ml | Astacus leptodactylus | - |
| 3.4.24.21 | digestive juice | 1 mg astacin/ml | Carcinus maenas | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.21 | Acetyl-Ala-Ala-Ala methylester + H2O | - |
Astacus astacus | ? | - |
? |  |
| 3.4.24.21 | Acetyl-Ala-Ala-Ala methylester + H2O | - |
Astacus leptodactylus | ? | - |
? | |
| 3.4.24.21 | Acetyl-Ala-Ala-Ala methylester + H2O | - |
Carcinus maenas | ? | - |
? | |
| 3.4.24.21 | azocasein + H2O | - |
Astacus astacus | ? | - |
? | |
| 3.4.24.21 | azocasein + H2O | - |
Astacus leptodactylus | ? | - |
? | |
| 3.4.24.21 | azocasein + H2O | - |
Carcinus maenas | ? | - |
? | |
| 3.4.24.21 | casein + H2O | heat-denatured form | Astacus astacus | hydrolyzed casein | - |
? | |
| 3.4.24.21 | casein + H2O | heat-denatured form | Astacus leptodactylus | hydrolyzed casein | - |
? | |
| 3.4.24.21 | casein + H2O | heat-denatured form | Carcinus maenas | hydrolyzed casein | - |
? | |
| 3.4.24.21 | additional information | No hydrolysis of carbobenzoxy-Ala-Ala, Nalpha-benzoyl-DL-Arg 2-naphthylamide (i.e. BANA), benzoyl-L-arginine ethyl ester, N-acetyl-L-tyrosine ethyl ester, Leu-Trp-Met-Arg-Phe-Ala, Gly-Pro-Gly-Gly-Pro-Ala | Astacus astacus | ? | - |
? | |
| 3.4.24.21 | additional information | No hydrolysis of carbobenzoxy-Ala-Ala, Nalpha-benzoyl-DL-Arg 2-naphthylamide (i.e. BANA), benzoyl-L-arginine ethyl ester, N-acetyl-L-tyrosine ethyl ester, Leu-Trp-Met-Arg-Phe-Ala, Gly-Pro-Gly-Gly-Pro-Ala | Astacus leptodactylus | ? | - |
? | |
| 3.4.24.21 | additional information | No hydrolysis of carbobenzoxy-Ala-Ala, Nalpha-benzoyl-DL-Arg 2-naphthylamide (i.e. BANA), benzoyl-L-arginine ethyl ester, N-acetyl-L-tyrosine ethyl ester, Leu-Trp-Met-Arg-Phe-Ala, Gly-Pro-Gly-Gly-Pro-Ala | Carcinus maenas | ? | - |
? | |
| 3.4.24.21 | Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O | - |
Astacus astacus | Succinyl-Ala-Ala-Ala + p-nitroaniline | - |
? | |
| 3.4.24.21 | Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O | - |
Astacus leptodactylus | Succinyl-Ala-Ala-Ala + p-nitroaniline | - |
? | |
| 3.4.24.21 | Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O | - |
Carcinus maenas | Succinyl-Ala-Ala-Ala + p-nitroaniline | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.21 | 30 | - |
resistant to self-degradation for several days at pH 8 | Astacus astacus |
| 3.4.24.21 | 30 | - |
resistant to self-degradation for several days at pH 8 | Astacus leptodactylus |
| 3.4.24.21 | 30 | - |
resistant to self-degradation for several days at pH 8 | Carcinus maenas |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.21 | additional information | - |
pI: 4 | Astacus astacus |
| 3.4.24.21 | additional information | - |
pI: 4 | Astacus leptodactylus |
| 3.4.24.21 | additional information | - |
pI: 4 | Carcinus maenas |
| 3.4.24.21 | 8 | - |
- |
Astacus astacus |
| 3.4.24.21 | 8 | - |
- |
Astacus leptodactylus |
| 3.4.24.21 | 8 | - |
- |
Carcinus maenas |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.21 | 4 | - |
below, rapid and irreversible inactivation | Astacus astacus |
| 3.4.24.21 | 4 | - |
below, rapid and irreversible inactivation | Astacus leptodactylus |
| 3.4.24.21 | 4 | - |
below, rapid and irreversible inactivation | Carcinus maenas |
| 3.4.24.21 | 8 | - |
resistant to self-degradation for several days at 30°C | Astacus astacus |
| 3.4.24.21 | 8 | - |
resistant to self-degradation for several days at 30°C | Astacus leptodactylus |
| 3.4.24.21 | 8 | - |
resistant to self-degradation for several days at 30°C | Carcinus maenas |
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