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Literature summary extracted from

  • Gomis-Ruth, F.X.; Grams, F.; Yiallouros, I.; Nar, H.; Kusthardt, U.; Zwilling, R.; Bode, W.; Stöcker, W.
    Crystal structures, spectroscopic features, and catalytic properties of cobalt(II), copper(II), nickel(II), and mercury(II) derivatives of the zinc endopeptidase astacin. A correlation of structure and proteolytic activity (1994), J. Biol. Chem., 269, 17111-17117.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.21 Astacus astacus Astacus astacus
3.4.24.21 X-ray crystallography of Cu(II)-astacin, Co(II)-astacin and Ni(II)-astacin Astacus astacus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.21 soluble
-
 Astacus astacus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.21 Co2+ activation of apoenzyme, can replace Zn2+ as catalytic ion yielding 140% of the activity of the zinc-containing enzyme, pentavalent metal coordination Astacus astacus
3.4.24.21 Cu2+ activation of apoenzyme, can replace Zn2+ as catalytic ion yielding 37% of the activity of the zinc-containing enzyme, pentavalent metal coordination Astacus astacus
3.4.24.21 additional information no apoenzyme activation by Ni2+ or Hg2+ with their octahedral and tetrahedral coordination Astacus astacus
3.4.24.21 Zn2+ requirement Astacus astacus
3.4.24.21 Zn2+ zinc metalloenzyme Astacus astacus
3.4.24.21 Zn2+ zinc-binding consensus sequence Astacus astacus
3.4.24.21 Zn2+ pentavalent metal coordination Astacus astacus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.21 Astacus astacus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.21 digestive juice
-
 Astacus astacus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.21 Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
-
 Astacus astacus Succinyl-Ala-Ala-Ala + p-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.21 25
-
 assay at Astacus astacus