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Literature summary extracted from

  • Nonaka, T.; Ishikawa, H.; Tsumuraya, Y.; Hashimoto, Y.; Dohmae, N.; Takio, K.
    Characterization of a thermostable lysine-specific metalloendopeptidase from the fruiting bodies of a basidiomycete, Grifola frondosa (1995), J. Biochem., 118, 1014-1020.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.20 1,10-phenanthroline strong, caseinolytic activity Grifola frondosa
3.4.24.20 2,2'-bipyridine strong, caseinolytic activity Grifola frondosa
3.4.24.20 2-mercaptoethanol weak, caseinolytic activity Grifola frondosa
3.4.24.20 6-aminohexanoic acid caseinolytic activity Grifola frondosa
3.4.24.20 agmatine caseinolytic activity Grifola frondosa
3.4.24.20 antipain weak, caseinolytic activity Grifola frondosa
3.4.24.20 Ba2+ 1 mM, weak, caseinolytic activity Grifola frondosa
3.4.24.20 Ca2+ 1 mM, weak, caseinolytic activity Grifola frondosa
3.4.24.20 Co2+ 0.1 mM, caseinolytic activity Grifola frondosa
3.4.24.20 Cu2+ 1 mM, caseinolytic activity Grifola frondosa
3.4.24.20 dithiothreitol caseinolytic activity; strong Grifola frondosa
3.4.24.20 DL-homoarginine caseinolytic activity Grifola frondosa
3.4.24.20 EDTA fully restorable by Ca2+; fully restorable by Zn2+, Mn2+, Co2+; strong, caseinolytic activity Grifola frondosa
3.4.24.20 Hg2+ strong, caseinolytic activity Grifola frondosa
3.4.24.20 L-aminobutyric acid caseinolytic activity Grifola frondosa
3.4.24.20 L-arginine caseinolytic activity Grifola frondosa
3.4.24.20 L-lysine caseinolytic activity Grifola frondosa
3.4.24.20 L-Lysine ethylester caseinolytic activity Grifola frondosa
3.4.24.20 L-ornithine caseinolytic activity Grifola frondosa
3.4.24.20 Mg2+ 1 mM, weak, caseinolytic activity Grifola frondosa
3.4.24.20 additional information no inhibition (of caseinolytic activity) by chymostatin, iodoacetate, leupeptin; PMSF; zinc acetate, elastatinal, Lys-Lys, 4 M urea Grifola frondosa
3.4.24.20 p-chloromercuribenzoate not Grifola frondosa
3.4.24.20 phosphoramidon weak, caseinolytic activity Grifola frondosa
3.4.24.20 tosyl-Lys chloromethyl ketone not Grifola frondosa
3.4.24.20 tosyl-Phe chloromethyl ketone weak, caseinolytic activity Grifola frondosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.20 cytosol
-
Grifola frondosa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.20 Ca2+ activation, zinc-depleted apoenzyme Grifola frondosa
3.4.24.20 Co2+ activation, zinc-depleted apoenzyme Grifola frondosa
3.4.24.20 Mn2+ activation, 0.1 mM, native enzyme and zinc-depleted apoenzyme Grifola frondosa
3.4.24.20 Zinc requirement, 1 atom per molecule of native enzyme Grifola frondosa
3.4.24.20 Zinc Zn2+ activates zinc-depleted apoenzyme Grifola frondosa
3.4.24.20 Zinc atomic absorption spectroscopy Grifola frondosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.20 20000
-
x * 20000, Grifola frondosa, SDS-PAGE Grifola frondosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.20 Grifola frondosa
-
i.e. Maitake, var. S.F.Gray
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.20
-
Grifola frondosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.20 fruit body
-
Grifola frondosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.20 additional information
-
-
Grifola frondosa

Storage Stability

EC Number Storage Stability Organism
3.4.24.20 -40°C, pH 7, at least a year Grifola frondosa
3.4.24.20 4°C, in various buffers, pH 5-10, about 20% loss of activity within 18 h Grifola frondosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.20 azocasein + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 Azocoll + H2O general protease substrate Grifola frondosa ?
-
?
3.4.24.20 Bovine serum albumin + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 Congo red elastin + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 Equine heart cytochrome c + H2O cleaves-X-Lys bonds Grifola frondosa ?
-
?
3.4.24.20 additional information specific cleavage of acyl-Lys bonds (-X-Lys-) in polypeptides Grifola frondosa ?
-
?
3.4.24.20 protamine sulfate + H2O
-
Grifola frondosa ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.20 ? x * 20000, Grifola frondosa, SDS-PAGE Grifola frondosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.20 37
-
assay at Grifola frondosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.20 50
-
3 h, about 30% loss of activity at pH 9 Grifola frondosa
3.4.24.20 60
-
3 h, about 55% loss of activity at pH 9 Grifola frondosa
3.4.24.20 70
-
and below, 3 h stable at pH 7.2 Grifola frondosa
3.4.24.20 70
-
3 h, about 90% loss of activity at pH 9 Grifola frondosa
3.4.24.20 70
-
3 h, about 40% loss of activity with Mn2+ instead of zinc Grifola frondosa
3.4.24.20 80
-
3 h, 40% loss of activity at pH 7.2, 30 min, about 90% loss of activity with Mn2+ instead of zinc Grifola frondosa
3.4.24.20 100
-
30 min, inactivation Grifola frondosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.20 additional information
-
pI: 7.46 Grifola frondosa
3.4.24.20 9
-
congo red elastin as substrate Grifola frondosa
3.4.24.20 9.5
-
azocoll or azocasein as substrate Grifola frondosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.20 6 10.5 more than 50% of maximal activity at pH 6 and 10.5 Grifola frondosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.20 5 10 18 h, about 20% loss of activity at 4°C Grifola frondosa
3.4.24.20 7.2
-
3 h, stable at 70°C and below, 40% loss of activity at 80°C, inactivation within 30 min at 100°C Grifola frondosa
3.4.24.20 9
-
3 h, about 30%, 55% and 90% loss of activity at 50°C, 60°C and 70°C, respectively Grifola frondosa