Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wolz, R.L.
    A kinetic comparison of the homologous proteases astacin and meprin A (1994), Arch. Biochem. Biophys., 310, 144-151.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.18 Acetyl-Arg-Pro-Gly-Tyr hydroxamate kinetics Mus musculus
3.4.24.18 actinonin i.e. 3-[[1-[[2-(hydroxymethyl)-1-pyrolidinyl]carbonyl]-2-methylpropyl]carbamoyl]octano hydroxamic acid, strong, kinetics Mus musculus
3.4.24.18 Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 Hydroxamyl-succinyl-Pro-Phe-Arg kinetics Mus musculus
3.4.24.21 Acetyl-Arg-Pro-Gly-Tyr-hydroxamate
-
 Astacus astacus
3.4.24.21 actinonin i.e. 3-[[1-[[2-(hydroxymethyl)-1-pyrolidinyl]carbonyl]-2-methylpropyl]carbamoyl]octano hydroxamic acid, weak, kinetics Astacus astacus
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg nitrobradykinin as substrate Astacus astacus
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg nitrobradykinin as substrate Astacus astacus
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg nitrobradykinin as substrate Astacus astacus
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg nitrobradykinin as substrate Astacus astacus
3.4.24.21 Hydroxamylsuccinyl-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 additional information most effective inhibitors are hydroxamates with aromatic side chains followed in order of decreasing affinity by those with side chains containing sulfur, acidic or basic groups Astacus astacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.18 0.174
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 0.182
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 0.183
-
 2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 0.22
-
 2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 0.226
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 0.29
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 0.296
-
 2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 0.331
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 0.402
-
 2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 1.22
-
 2-Aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 1.38
-
 2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 2.46
-
 2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.21 0.029
-
 2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 0.044
-
 2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 0.085
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 0.116
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 0.154
-
 2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 0.194
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 0.209
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 0.281
-
 2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 0.306
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 0.327
-
 2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 0.867 0.87 2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 0.954
-
 2-Aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.21 Zn2+ requirement Astacus astacus
3.4.24.21 Zn2+ zinc metalloenzyme Astacus astacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.18 additional information
-
 amino acid sequence of protease domain of meprin A compared to that of meprin B, EC 3.4.24.63 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.18 Mus musculus
-
-
-
3.4.24.21 Astacus astacus
-
 i.e. Astacus fluviatilis
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.18 kidney
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.18 additional information
-
-
 Mus musculus
3.4.24.18 0.00363
-
 succinyl-Ala-Ala-Ala 4-nitroanilide Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.18 2-aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser Mus musculus 2-aminobenzoyl-Arg-Gly-Pro-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.18 2-aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser Mus musculus 2-aminobenzoyl-Arg-Hyp-Gly-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.18 2-aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Ala-Ser Mus musculus 2-aminobenzoyl-Arg-Pro-Gly-Ala + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.18 2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Glu-Ser Mus musculus 2-aminobenzoyl-Arg-Pro-Gly-Glu + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.18 2-aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage sites: Leu-Ser (major site) and Gly-Leu Mus musculus 2-aminobenzoyl-Arg-Pro-Gly-Leu + Ser-Pro-(4-nitro)Phe-Arg major products ?
3.4.24.18 2-aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage sites: Gly-Lys (major site) and Lys-Ser Mus musculus 2-aminobenzoyl-Arg-Pro-Gly + Lys-Ser-Pro-(4-nitro)Phe-Arg major products ?
3.4.24.18 2-aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrates, cleavage site: Phe-Ser Mus musculus 2-aminobenzoyl-Arg-Pro-Ile-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.18 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg + H2O chromogenic bradykinin analog Mus musculus Arg-Pro-Pro-Gly-(4-nitro)Phe + Ala-Pro-Phe-Arg
-
 ?
3.4.24.18 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg + H2O chromogenic bradykinin analog Mus musculus Arg-Pro-Pro-Gly-(4-nitro)Phe + Arg-Pro-Phe-Arg
-
 ?
3.4.24.18 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg + H2O chromogenic bradykinin analog Mus musculus Arg-Pro-Pro-Gly-(4-nitro)Phe + Lys-Pro-Phe-Arg
-
 ?
3.4.24.18 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg + H2O chromogenic bradykinin analog Mus musculus Arg-Pro-Pro-Gly-(4-nitro)Phe + Phe-Pro-Phe-Arg
-
 ?
3.4.24.18 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg + H2O i.e. nitrobradykinin, chromogenic bradykinin analog Mus musculus Arg-Pro-Pro-Gly-(4-nitro)Phe + Ser-Pro-Phe-Arg
-
 ?
3.4.24.18 azocasein + H2O
-
 Mus musculus fragments of azocasein
-
 ?
3.4.24.18 bradykinin + H2O one cleavage site: Phe-Ser Mus musculus Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg
-
 ?
3.4.24.18 Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O arylamidolysis Mus musculus ?
-
 ?
3.4.24.21 2-aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser Astacus astacus 2-aminobenzoyl-Arg-Gly-Pro-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.21 2-aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser Astacus astacus 2-aminobenzoyl-Arg-Hyp-Gly-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.21 2-aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage sites: Gly-Ala and Ala-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Gly-Ala + Ser-Pro-(4-nitro)Phe-Arg major products ?
3.4.24.21 2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Glu-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Gly-Glu + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.21 2-aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Leu-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Gly-Leu + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.21 2-aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Lys-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Gly-Lys + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.21 2-aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O best of fluorogenic bradykinin analog substrates, cleavage site: Phe-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Ile-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg + H2O chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Ala-Pro-Phe-Arg
-
 ?
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg + H2O chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Arg-Pro-Phe-Arg
-
 ?
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg + H2O chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Lys-Pro-Phe-Arg
-
 ?
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg + H2O chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Phe-Pro-Phe-Arg
-
 ?
3.4.24.21 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg + H2O i.e. nitrobradykinin, chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Ser-Pro-Phe-Arg
-
 ?
3.4.24.21 azocasein + H2O
-
 Astacus astacus ?
-
 ?
3.4.24.21 bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg Astacus astacus Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
-
 ?
3.4.24.21 additional information No hydrolysis of Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg Astacus astacus ?
-
 ?
3.4.24.21 Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
-
 Astacus astacus Succinyl-Ala-Ala-Ala + p-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.18 30
-
 assay at Mus musculus
3.4.24.21 30
-
 assay at Astacus astacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.18 1.1
-
 2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 2.4
-
 2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 4.9
-
 2-Aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 5
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 7.6
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 12
-
 2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 19.6
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 26.7
-
 2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 40.9
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 51.5
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
-
 Mus musculus
3.4.24.18 98.5
-
 2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.18 133
-
 2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Mus musculus
3.4.24.21 0.001
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 0.002
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 0.004
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 2.8
-
 2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-4-nitrophenylalanine-Arg
-
 Astacus astacus
3.4.24.21 3.6
-
 2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 7.1
-
 2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 14.3
-
 2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 15.1
-
 2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 44.4
-
 2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 78.4
-
 2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
3.4.24.21 78.7
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
-
 Astacus astacus
3.4.24.21 834
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
-
 Astacus astacus