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Literature summary extracted from

  • Kervinen, J.; Törmäkangas, K.; Runeberg-Roos, P.; Guruprasad, K.; Blundell, T.; Teeri, T.H.
    Structure and possible function of aspartic proteinases in barley and other plants (1995), Adv. Exp. Med. Biol., 362, 241-254.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.40 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Triticum aestivum
3.4.23.40 Diazoacetylnorleucine methyl ester
-
 Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.40 vacuole
-
 Hordeum vulgare 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.40 16000
-
 1 * 34000 + 1 * 16000, SDS-PAGE Theobroma cacao
3.4.23.40 20000
-
 ultracentirfugation Cannabis sativa
3.4.23.40 28000
-
 gel filtration Fagopyrum esculentum
3.4.23.40 34000
-
 1 * 34000 + 1 * 16000, SDS-PAGE Theobroma cacao
3.4.23.40 36000 37000 ultracentrifugation, gel filtration Nelumbo nucifera
3.4.23.40 36000 40000 gel filtration Nicotiana tabacum
3.4.23.40 37000
-
 1 * 37000, SDS-PAGE Solanum lycopersicum
3.4.23.40 40000
-
 2 * 40000, sequence of cDNA Hordeum vulgare
3.4.23.40 40000 42000 gel filtration Cucumis sativus
3.4.23.40 40000 42000 gel filtration Cucurbita maxima
3.4.23.40 45000
-
-
 Theobroma cacao
3.4.23.40 48000
-
 calculation from sequence of cDNA Oryza sativa
3.4.23.40 48000
-
 2 * 48000, SDS-PAGE Hordeum vulgare
3.4.23.40 50000
-
 seeds, gel filtration Triticum aestivum
3.4.23.40 58000
-
 1 * 58000, SDS-PAGE Triticum aestivum
3.4.23.40 59000
-
 gel filtration Nepenthes macfarlanei
3.4.23.40 60000 65000 gel filtration Oryza sativa
3.4.23.40 66000
-
 gel filtration Triticum durum x Haynaldia villosa
3.4.23.40 89000
-
 leaf, gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.40 Protein + H2O Triticum aestivum
-
 ?
-
 ?
3.4.23.40 Protein + H2O Brassica napus
-
 ?
-
 ?
3.4.23.40 Protein + H2O Cucumis sativus
-
 ?
-
 ?
3.4.23.40 Protein + H2O Pinus sylvestris
-
 ?
-
 ?
3.4.23.40 Protein + H2O Sorghum bicolor
-
 ?
-
 ?
3.4.23.40 Protein + H2O Oryza sativa
-
 ?
-
 ?
3.4.23.40 Protein + H2O Cucurbita maxima
-
 ?
-
 ?
3.4.23.40 Protein + H2O Fagopyrum esculentum
-
 ?
-
 ?
3.4.23.40 Protein + H2O Pinus banksiana
-
 ?
-
 ?
3.4.23.40 Protein + H2O Triticum durum x Haynaldia villosa
-
 ?
-
 ?
3.4.23.40 Protein + H2O Cannabis sativa
-
 ?
-
 ?
3.4.23.40 Protein + H2O Nelumbo nucifera
-
 ?
-
 ?
3.4.23.40 Protein + H2O Cynara cardunculus
-
 ?
-
 ?
3.4.23.40 Protein + H2O Theobroma cacao
-
 ?
-
 ?
3.4.23.40 Protein + H2O Drosera peltata
-
 ?
-
 ?
3.4.23.40 Protein + H2O Hordeum vulgare may participate in the processing of the C-terminus of barley prolectin ?
-
 ?
3.4.23.40 Protein + H2O Nicotiana tabacum hydrolysis of pathogenesis-related proteins ?
-
 ?
3.4.23.40 Protein + H2O Solanum lycopersicum hydrolysis of pathogenesis-related proteins ?
-
 ?
3.4.23.40 Protein + H2O Nepenthes macfarlanei contribution to the digestion of trapped insects ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.40 Brassica napus
-
 rape
-
3.4.23.40 Cannabis sativa
-
 hemp
-
3.4.23.40 Cucumis sativus
-
 cucumber
-
3.4.23.40 Cucurbita maxima
-
 squash
-
3.4.23.40 Cynara cardunculus
-
 cardoon
-
3.4.23.40 Drosera peltata
-
-
-
3.4.23.40 Fagopyrum esculentum
-
 buckwheat
-
3.4.23.40 Hordeum vulgare
-
 L. Cv. Kustaa, barley
-
3.4.23.40 Nelumbo nucifera
-
 lotus
-
3.4.23.40 Nepenthes macfarlanei
-
-
-
3.4.23.40 Nicotiana tabacum
-
 tobacco
-
3.4.23.40 Oryza sativa
-
 rice
-
3.4.23.40 Pinus banksiana
-
 Jack pine
-
3.4.23.40 Pinus sylvestris
-
 Scots pine
-
3.4.23.40 Solanum lycopersicum
-
 tomato
-
3.4.23.40 Sorghum bicolor
-
 sorghum
-
3.4.23.40 Theobroma cacao
-
 cacao
-
3.4.23.40 Triticum aestivum
-
 wheat
-
3.4.23.40 Triticum durum x Haynaldia villosa
-
 hybrid between Triticum durum and Hynaldia villosa
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.40 cotyledon
-
 Nelumbo nucifera
-
3.4.23.40 flower
-
 Cynara cardunculus
-
3.4.23.40 leaf
-
 Triticum aestivum
-
3.4.23.40 leaf
-
 Hordeum vulgare
-
3.4.23.40 leaf
-
 Nicotiana tabacum
-
3.4.23.40 leaf
-
 Solanum lycopersicum
-
3.4.23.40 pitcher secretion
-
 Nepenthes macfarlanei
-
3.4.23.40 seed
-
 Triticum aestivum
-
3.4.23.40 seed
-
 Brassica napus
-
3.4.23.40 seed
-
 Cucumis sativus
-
3.4.23.40 seed
-
 Pinus sylvestris
-
3.4.23.40 seed
-
 Sorghum bicolor
-
3.4.23.40 seed
-
 Cucurbita maxima
-
3.4.23.40 seed
-
 Fagopyrum esculentum
-
3.4.23.40 seed
-
 Pinus banksiana
-
3.4.23.40 seed
-
 Triticum durum x Haynaldia villosa
-
3.4.23.40 seed
-
 Cannabis sativa
-
3.4.23.40 seed
-
 Nelumbo nucifera
-
3.4.23.40 seed
-
 Theobroma cacao
-
3.4.23.40 seed embryo, aleurone layer Oryza sativa
-
3.4.23.40 seed developing, resting and germinating grains, in the embryo, aleurone layer, testa and pericarp Hordeum vulgare
-
3.4.23.40 stem
-
 Drosera peltata
-
3.4.23.40 trapping leaf
-
 Drosera peltata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.40 Albumin + H2O
-
 Triticum aestivum ?
-
 ?
3.4.23.40 Albumin + H2O
-
 Sorghum bicolor ?
-
 ?
3.4.23.40 Albumin + H2O
-
 Fagopyrum esculentum ?
-
 ?
3.4.23.40 Albumin + H2O
-
 Nepenthes macfarlanei ?
-
 ?
3.4.23.40 Albumin + H2O
-
 Theobroma cacao ?
-
 ?
3.4.23.40 casein + H2O
-
 Brassica napus ?
-
 ?
3.4.23.40 casein + H2O
-
 Cucumis sativus ?
-
 ?
3.4.23.40 Edestin + H2O
-
 Cannabis sativa ?
-
 ?
3.4.23.40 Fibrin + H2O
-
 Nepenthes macfarlanei ?
-
 ?
3.4.23.40 Gliadin + H2O
-
 Triticum aestivum ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Triticum aestivum ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Nicotiana tabacum ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Solanum lycopersicum ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Cucumis sativus ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Pinus sylvestris ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Sorghum bicolor ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Cucurbita maxima ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Pinus banksiana ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Triticum durum x Haynaldia villosa ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Cannabis sativa ?
-
 ?
3.4.23.40 Hemoglobin + H2O
-
 Nelumbo nucifera ?
-
 ?
3.4.23.40 Protein + H2O
-
 Triticum aestivum ?
-
 ?
3.4.23.40 Protein + H2O
-
 Brassica napus ?
-
 ?
3.4.23.40 Protein + H2O
-
 Cucumis sativus ?
-
 ?
3.4.23.40 Protein + H2O
-
 Pinus sylvestris ?
-
 ?
3.4.23.40 Protein + H2O
-
 Sorghum bicolor ?
-
 ?
3.4.23.40 Protein + H2O
-
 Oryza sativa ?
-
 ?
3.4.23.40 Protein + H2O
-
 Cucurbita maxima ?
-
 ?
3.4.23.40 Protein + H2O
-
 Fagopyrum esculentum ?
-
 ?
3.4.23.40 Protein + H2O
-
 Pinus banksiana ?
-
 ?
3.4.23.40 Protein + H2O
-
 Triticum durum x Haynaldia villosa ?
-
 ?
3.4.23.40 Protein + H2O
-
 Cannabis sativa ?
-
 ?
3.4.23.40 Protein + H2O
-
 Nelumbo nucifera ?
-
 ?
3.4.23.40 Protein + H2O
-
 Cynara cardunculus ?
-
 ?
3.4.23.40 Protein + H2O
-
 Theobroma cacao ?
-
 ?
3.4.23.40 Protein + H2O
-
 Drosera peltata ?
-
 ?
3.4.23.40 Protein + H2O may participate in the processing of the C-terminus of barley prolectin Hordeum vulgare ?
-
 ?
3.4.23.40 Protein + H2O hydrolysis of pathogenesis-related proteins Nicotiana tabacum ?
-
 ?
3.4.23.40 Protein + H2O hydrolysis of pathogenesis-related proteins Solanum lycopersicum ?
-
 ?
3.4.23.40 Protein + H2O contribution to the digestion of trapped insects Nepenthes macfarlanei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.40 dimer 2 * 48000, SDS-PAGE Hordeum vulgare
3.4.23.40 dimer 2 * 40000, sequence of cDNA Hordeum vulgare
3.4.23.40 dimer 2 * 46000-49000, SDS-PAGE, sequence of cDNA Cynara cardunculus
3.4.23.40 dimer 1 * 34000 + 1 * 16000, SDS-PAGE Theobroma cacao
3.4.23.40 monomer 1 * 58000, SDS-PAGE Triticum aestivum
3.4.23.40 monomer 1 * 37000, SDS-PAGE Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.4.23.40 Nepenthesin
-
 Nepenthes macfarlanei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.40 2 3 substrate casein Drosera peltata
3.4.23.40 2.2
-
 substrates albumin, fibrin Nepenthes macfarlanei
3.4.23.40 2.4 3.8 substrates casein, hemoglobin Nelumbo nucifera
3.4.23.40 2.5 3.5 substrate hemoglobin Solanum lycopersicum
3.4.23.40 2.5 4.2 substrates casein, hemoglobin Oryza sativa
3.4.23.40 3
-
 substrate hemoglobin Nicotiana tabacum
3.4.23.40 3
-
 substrate albumin Triticum aestivum
3.4.23.40 3.1
-
 chromophoric peptide substrate Triticum durum x Haynaldia villosa
3.4.23.40 3.2
-
 substrate hemoglobin Cucumis sativus
3.4.23.40 3.4
-
 substrate hemoglobin Cannabis sativa
3.4.23.40 3.4
-
 substrate albumin Theobroma cacao
3.4.23.40 3.5
-
 substrate hemoglobin Pinus banksiana
3.4.23.40 3.5
-
 substrate albumin Brassica napus
3.4.23.40 3.5
-
 substrate albumin Fagopyrum esculentum
3.4.23.40 3.5 3.9 substrate hemoglobin Hordeum vulgare
3.4.23.40 3.6
-
 substrate hemoglobin Cucurbita maxima
3.4.23.40 3.6
-
 substrate albumin Sorghum bicolor
3.4.23.40 3.7
-
 substrate hemoglobin Pinus sylvestris
3.4.23.40 3.7 4.1 chromophoric peptide substrate Hordeum vulgare
3.4.23.40 4.3
-
 substrate edestin Cannabis sativa
3.4.23.40 4.5
-
 substrate gliadin Triticum aestivum
3.4.23.40 5.1
-
 substrate casein Cynara cardunculus