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Literature summary extracted from
- Procathepsin E and cathepsin E (1995), Methods Enzymol., 248, 120-136.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.34 | guinea pig | Cavia porcellus |
| 3.4.23.34 | guinea pig | Homo sapiens |
| 3.4.23.34 | guinea pig | Rattus norvegicus |
| 3.4.23.34 | guinea pig | Oryctolagus cuniculus |
| 3.4.23.34 | human | Cavia porcellus |
| 3.4.23.34 | human | Homo sapiens |
| 3.4.23.34 | human | Rattus norvegicus |
| 3.4.23.34 | human | Oryctolagus cuniculus |
| 3.4.23.34 | rabbit | Cavia porcellus |
| 3.4.23.34 | rabbit | Homo sapiens |
| 3.4.23.34 | rabbit | Rattus norvegicus |
| 3.4.23.34 | rabbit | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.34 | 1,2-epoxy-3-(p-nitrophenoxy)propane | ir | Cavia porcellus |  |
| 3.4.23.34 | 1,2-epoxy-3-(p-nitrophenoxy)propane | ir | Homo sapiens | |
| 3.4.23.34 | 1,2-epoxy-3-(p-nitrophenoxy)propane | ir | Oryctolagus cuniculus | |
| 3.4.23.34 | 1,2-epoxy-3-(p-nitrophenoxy)propane | ir | Rattus norvegicus | |
| 3.4.23.34 | Diazoacetyl-DL-norleucine methyl ester | ir | Cavia porcellus | |
| 3.4.23.34 | Diazoacetyl-DL-norleucine methyl ester | ir | Homo sapiens | |
| 3.4.23.34 | Diazoacetyl-DL-norleucine methyl ester | ir | Oryctolagus cuniculus | |
| 3.4.23.34 | Diazoacetyl-DL-norleucine methyl ester | ir | Rattus norvegicus | |
| 3.4.23.34 | pepstatin | - |
Cavia porcellus | |
| 3.4.23.34 | pepstatin | - |
Homo sapiens | |
| 3.4.23.34 | pepstatin | - |
Oryctolagus cuniculus | |
| 3.4.23.34 | pepstatin | - |
Rattus norvegicus | |
| 3.4.23.34 | Protein inhibitor from Ascaris lumbricoides | MW 17000 | Cavia porcellus | |
| 3.4.23.34 | Protein inhibitor from Ascaris lumbricoides | MW 17000 | Homo sapiens | |
| 3.4.23.34 | Protein inhibitor from Ascaris lumbricoides | MW 17000 | Oryctolagus cuniculus | |
| 3.4.23.34 | Protein inhibitor from Ascaris lumbricoides | MW 17000 | Rattus norvegicus | |
| 3.4.23.34 | Tripeptide analogs | - |
Cavia porcellus | |
| 3.4.23.34 | Tripeptide analogs | - |
Homo sapiens | |
| 3.4.23.34 | Tripeptide analogs | - |
Oryctolagus cuniculus | |
| 3.4.23.34 | Tripeptide analogs | - |
Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.34 | 0.0023 | - |
Substance P | Arg-modified, pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.0034 | - |
renin | porcine substrate, pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.0039 | - |
Substance P | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.0059 | - |
Eledoisin | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.0071 | - |
[Tyr8]-substance P | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.009 | - |
big ET-1 | - |
Homo sapiens | |
| 3.4.23.34 | 0.009 | - |
big ET-1 | pH 4.3 | Cavia porcellus | |
| 3.4.23.34 | 0.0093 | - |
Acetyl-substance P | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.011 | - |
[His10]-substance P | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.024 | - |
Substance P(4-11) | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.025 | - |
Neurokinin A | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.03 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5 | Cavia porcellus | |
| 3.4.23.34 | 0.042 | - |
Substance P(2-11) | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.07 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5 | Cavia porcellus | |
| 3.4.23.34 | 0.083 | - |
Acetyl-substance P(2-11) | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.143 | - |
acidic fibroblast growth factor | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 0.145 | - |
Pro-Thr-Glu-Phe-(4-nitro)Phe-Arg-Leu | pH 3.1 | Cavia porcellus | |
| 3.4.23.34 | 0.16 | - |
Lys-Pro-Ala-Glu-Phe-(4-nitro)Phe-Arg-Leu | pH 3.1 | Cavia porcellus | |
| 3.4.23.34 | 0.4 | - |
Acetyl-substance P(3-11) | - |
Cavia porcellus | |
| 3.4.23.34 | 0.5 | - |
Substance P(1-9) | value above | Cavia porcellus | |
| 3.4.23.34 | 0.66 | - |
Substance P(3-11) | - |
Cavia porcellus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.23.34 | cytosol | - |
Cavia porcellus | 5829 | - |
| 3.4.23.34 | cytosol | - |
Homo sapiens | 5829 | - |
| 3.4.23.34 | cytosol | - |
Rattus norvegicus | 5829 | - |
| 3.4.23.34 | cytosol | - |
Oryctolagus cuniculus | 5829 | - |
| 3.4.23.34 | membrane | - |
Cavia porcellus | 16020 | - |
| 3.4.23.34 | membrane | - |
Homo sapiens | 16020 | - |
| 3.4.23.34 | membrane | - |
Rattus norvegicus | 16020 | - |
| 3.4.23.34 | membrane | - |
Oryctolagus cuniculus | 16020 | - |
| 3.4.23.34 | soluble | - |
Cavia porcellus | - |
- |
| 3.4.23.34 | soluble | - |
Homo sapiens | - |
- |
| 3.4.23.34 | soluble | - |
Rattus norvegicus | - |
- |
| 3.4.23.34 | soluble | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.34 | additional information | - |
amino acid composition and sequence of procathepsin E from rabbit, guinea pig and human | Cavia porcellus |
| 3.4.23.34 | additional information | - |
amino acid composition and sequence of procathepsin E from rabbit, guinea pig and human | Homo sapiens |
| 3.4.23.34 | additional information | - |
amino acid composition and sequence of procathepsin E from rabbit, guinea pig and human | Rattus norvegicus |
| 3.4.23.34 | additional information | - |
amino acid composition and sequence of procathepsin E from rabbit, guinea pig and human | Oryctolagus cuniculus |
| 3.4.23.34 | 39000 | - |
2 * 39000, SDS-PAGE, reducing conditions | Cavia porcellus |
| 3.4.23.34 | 76000 | - |
guinea pig, SDS-PAGE, non-reducing conditions | Cavia porcellus |
| 3.4.23.34 | 76000 | - |
guinea pig, SDS-PAGE, non-reducing conditions | Homo sapiens |
| 3.4.23.34 | 82000 | - |
guinea pig, procathepsin E, SDS-PAGE, non-reducing conditions | Cavia porcellus |
| 3.4.23.34 | 82000 | - |
guinea pig, procathepsin E, SDS-PAGE, non-reducing conditions | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.34 | Cavia porcellus | - |
- |
- |
| 3.4.23.34 | Homo sapiens | - |
- |
- |
| 3.4.23.34 | Oryctolagus cuniculus | - |
- |
- |
| 3.4.23.34 | Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.23.34 | glycoprotein | - |
Homo sapiens |
| 3.4.23.34 | glycoprotein | - |
Rattus norvegicus |
| 3.4.23.34 | glycoprotein | - |
Oryctolagus cuniculus |
| 3.4.23.34 | glycoprotein | carbohydrates account for 3-4% of total molecular mass | Cavia porcellus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.34 | - |
Homo sapiens |
| 3.4.23.34 | - |
Oryctolagus cuniculus |
| 3.4.23.34 | as procathepsin E | Cavia porcellus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.23.34 | blood | - |
Cavia porcellus | - |
| 3.4.23.34 | blood | - |
Homo sapiens | - |
| 3.4.23.34 | blood | - |
Rattus norvegicus | - |
| 3.4.23.34 | blood | - |
Oryctolagus cuniculus | - |
| 3.4.23.34 | colon | - |
Cavia porcellus | - |
| 3.4.23.34 | colon | - |
Homo sapiens | - |
| 3.4.23.34 | colon | - |
Rattus norvegicus | - |
| 3.4.23.34 | colon | - |
Oryctolagus cuniculus | - |
| 3.4.23.34 | erythrocyte | - |
Homo sapiens | - |
| 3.4.23.34 | erythrocyte | - |
Rattus norvegicus | - |
| 3.4.23.34 | gastric mucosa | - |
Cavia porcellus | - |
| 3.4.23.34 | gastric mucosa | - |
Homo sapiens | - |
| 3.4.23.34 | gastric mucosa | - |
Rattus norvegicus | - |
| 3.4.23.34 | gastric mucosa | - |
Oryctolagus cuniculus | - |
| 3.4.23.34 | liver | fetus | Cavia porcellus | - |
| 3.4.23.34 | liver | fetus | Homo sapiens | - |
| 3.4.23.34 | liver | fetus | Rattus norvegicus | - |
| 3.4.23.34 | liver | fetus | Oryctolagus cuniculus | - |
| 3.4.23.34 | additional information | tissue distribution | Cavia porcellus | - |
| 3.4.23.34 | additional information | tissue distribution | Homo sapiens | - |
| 3.4.23.34 | additional information | tissue distribution | Rattus norvegicus | - |
| 3.4.23.34 | additional information | tissue distribution | Oryctolagus cuniculus | - |
| 3.4.23.34 | neutrophil | rat | Rattus norvegicus | - |
| 3.4.23.34 | neutrophil | not human | Homo sapiens | - |
| 3.4.23.34 | ovarian cancer cell | - |
Cavia porcellus | - |
| 3.4.23.34 | ovarian cancer cell | - |
Homo sapiens | - |
| 3.4.23.34 | ovarian cancer cell | - |
Rattus norvegicus | - |
| 3.4.23.34 | ovarian cancer cell | - |
Oryctolagus cuniculus | - |
| 3.4.23.34 | ovary | mucinous tumors | Cavia porcellus | - |
| 3.4.23.34 | ovary | mucinous tumors | Homo sapiens | - |
| 3.4.23.34 | ovary | mucinous tumors | Rattus norvegicus | - |
| 3.4.23.34 | ovary | mucinous tumors | Oryctolagus cuniculus | - |
| 3.4.23.34 | spleen | - |
Rattus norvegicus | - |
| 3.4.23.34 | stomach | - |
Cavia porcellus | - |
| 3.4.23.34 | stomach | - |
Homo sapiens | - |
| 3.4.23.34 | stomach | - |
Rattus norvegicus | - |
| 3.4.23.34 | stomach | - |
Oryctolagus cuniculus | - |
| 3.4.23.34 | thymus | - |
Cavia porcellus | - |
| 3.4.23.34 | thymus | - |
Homo sapiens | - |
| 3.4.23.34 | thymus | - |
Rattus norvegicus | - |
| 3.4.23.34 | thymus | - |
Oryctolagus cuniculus | - |
| 3.4.23.34 | urinary bladder | - |
Cavia porcellus | - |
| 3.4.23.34 | urinary bladder | - |
Homo sapiens | - |
| 3.4.23.34 | urinary bladder | - |
Rattus norvegicus | - |
| 3.4.23.34 | urinary bladder | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.34 | 2.2 | - |
hemoglobin as substrate, calculated on the basis of leucine equivalents released | Cavia porcellus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.23.34 | -20°C, procathepsin E, in neutral or weakly alkaline solution with 50% glycerol, several months, cathepsin E is stable under the same conditions provided the pH-value of the storage solution is kept around pH 5.5 | Cavia porcellus |
| 3.4.23.34 | 4°C, procathepsin E, stable in saturated solution of ammonium sulfate adjusted to neutral or weakly alkaline pH, cathepsin E is stable under the same conditions provided the pH-value of the storage solution is kept around pH 5.5 | Cavia porcellus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.34 | Acetyl-substance P + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Acetyl-substance P(2-11) + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Acetyl-substance P(3-11) + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | acidic fibroblast growth factor + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Acidic fibroblast growth factor fragment 102-111 + H2O | i.e. His-Ala-Glu-Lys-His-Trp-Phe-Val-Gly-Leu or acidic FGF 102-111 | Cavia porcellus | His-Ala-Glu-Lys-His-Trp-Phe + Val-Gly-Leu | - |
? | |
| 3.4.23.34 | Acidic fibroblast growth factor fragment 102-111 + H2O | i.e. His-Ala-Glu-Lys-His-Trp-Phe-Val-Gly-Leu or acidic FGF 102-111 | Oryctolagus cuniculus | His-Ala-Glu-Lys-His-Trp-Phe + Val-Gly-Leu | - |
? | |
| 3.4.23.34 | Arg-modified substance P + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Basic fibroblast growth factor fragment 106-120 + H2O | i.e. Tyr-Arg-Ser-Arg-Lys-Tyr-Ser-Ser-Trp-Tyr-Val-Ala-Leu-Lys-Arg or basic FGF 106-120, major cleavage site: Tyr-Val, minor site: Trp-Tyr | Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Basic fibroblast growth factor fragment 106-120 + H2O | i.e. Tyr-Arg-Ser-Arg-Lys-Tyr-Ser-Ser-Trp-Tyr-Val-Ala-Leu-Lys-Arg or basic FGF 106-120, major cleavage site: Tyr-Val, minor site: Trp-Tyr | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.23.34 | big ET-1 + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | big ET-1 + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.23.34 | Bovine gamma-globulin + H2O | - |
Cavia porcellus | Hydrolyzed bovine gamma-globulin | - |
? | |
| 3.4.23.34 | Bovine gamma-globulin + H2O | - |
Rattus norvegicus | Hydrolyzed bovine gamma-globulin | - |
? | |
| 3.4.23.34 | Bovine serum albumin + H2O | - |
Cavia porcellus | Hydrolyzed bovine serum albumin | - |
? | |
| 3.4.23.34 | Bovine serum albumin + H2O | - |
Homo sapiens | Hydrolyzed bovine serum albumin | - |
? | |
| 3.4.23.34 | Bovine serum albumin + H2O | - |
Rattus norvegicus | Hydrolyzed bovine serum albumin | - |
? | |
| 3.4.23.34 | casein + H2O | - |
Cavia porcellus | hydrolyzed casein | - |
? | |
| 3.4.23.34 | casein + H2O | - |
Homo sapiens | hydrolyzed casein | - |
? | |
| 3.4.23.34 | casein + H2O | - |
Rattus norvegicus | hydrolyzed casein | - |
? | |
| 3.4.23.34 | Cholecystokinin 8 + H2O | i.e. Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2, cleavage site: Trp-Met | Cavia porcellus | Asp-Tyr-Met-Gly-Trp + Met-Asp-Phe-NH2 | - |
? | |
| 3.4.23.34 | Cholecystokinin 8 + H2O | i.e. Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2, cleavage site: Trp-Met | Oryctolagus cuniculus | Asp-Tyr-Met-Gly-Trp + Met-Asp-Phe-NH2 | - |
? | |
| 3.4.23.34 | dynorphin A + H2O | cleavage site: Phe4-Leu5 | Cavia porcellus | hydrolyzed dynorphin A | - |
? | |
| 3.4.23.34 | dynorphin A + H2O | cleavage site: Phe4-Leu5 | Oryctolagus cuniculus | hydrolyzed dynorphin A | - |
? | |
| 3.4.23.34 | Egg albumin + H2O | i.e. ovalbumin | Cavia porcellus | Hydrolyzed egg albumin | - |
? | |
| 3.4.23.34 | Eledoisin + H2O | i.e. pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe-Ile-Gly-Leu-Met-NH2, cleavage site: Phe-Ile | Cavia porcellus | Pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe + Ile-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Eledoisin + H2O | i.e. pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe-Ile-Gly-Leu-Met-NH2, cleavage site: Phe-Ile | Oryctolagus cuniculus | Pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe + Ile-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Hemoglobin + H2O | - |
Cavia porcellus | Hydrolyzed hemoglobin | - |
? | |
| 3.4.23.34 | Hemoglobin + H2O | - |
Homo sapiens | Hydrolyzed hemoglobin | - |
? | |
| 3.4.23.34 | Hemoglobin + H2O | - |
Rattus norvegicus | Hydrolyzed hemoglobin | - |
? | |
| 3.4.23.34 | Human beta-endorphin + H2O | major cleavage site: Leu17-Phe18, minor site: Thr16-Leu17 | Cavia porcellus | Hydrolyzed human beta-endorphin | - |
? | |
| 3.4.23.34 | Human beta-endorphin + H2O | major cleavage site: Leu17-Phe18, minor site: Thr16-Leu17 | Oryctolagus cuniculus | Hydrolyzed human beta-endorphin | - |
? | |
| 3.4.23.34 | Human endothelin precursor big ET-1 + H2O | cleavage site: Trp-Val | Cavia porcellus | Human endothelin precursor ET-1 + respective C-terminal fragment | - |
? | |
| 3.4.23.34 | Human endothelin precursor big ET-1 + H2O | cleavage site: Trp-Val | Homo sapiens | Human endothelin precursor ET-1 + respective C-terminal fragment | - |
? | |
| 3.4.23.34 | Human endothelin precursor big ET-1 + H2O | cleavage site: Trp-Val | Oryctolagus cuniculus | Human endothelin precursor ET-1 + respective C-terminal fragment | - |
? | |
| 3.4.23.34 | Human renin substrate + H2O | i.e. angiotensinogen fragment 1-13 or Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Val-Ile-His, cleavage site: Leu-Val | Cavia porcellus | Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Val-Ile-His | - |
? | |
| 3.4.23.34 | Human renin substrate + H2O | i.e. angiotensinogen fragment 1-13 or Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Val-Ile-His, cleavage site: Leu-Val | Oryctolagus cuniculus | Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Val-Ile-His | - |
? | |
| 3.4.23.34 | Kassinin + H2O | i.e. Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Phe-Val | Cavia porcellus | Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe + Val-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Kassinin + H2O | i.e. Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Phe-Val | Oryctolagus cuniculus | Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe + Val-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Lys-Pro-Ala-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Cavia porcellus | Lys-Pro-Ala-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
| 3.4.23.34 | Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | synthetic chromogenic peptide, less suitable peptide substrate than substance P or other tachykinins | Cavia porcellus | Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
| 3.4.23.34 | Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | synthetic chromogenic peptide, less suitable peptide substrate than substance P or other tachykinins | Homo sapiens | Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
| 3.4.23.34 | Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | synthetic chromogenic peptide, less suitable peptide substrate than substance P or other tachykinins | Rattus norvegicus | Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
| 3.4.23.34 | Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | synthetic chromogenic peptide, less suitable peptide substrate than substance P or other tachykinins | Oryctolagus cuniculus | Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
| 3.4.23.34 | Neurokinin A + H2O | i.e. His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Phe-Val | Cavia porcellus | His-Lys-Thr-Asp-Ser-Phe + Val-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Neurokinin A + H2O | i.e. His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Phe-Val | Oryctolagus cuniculus | His-Lys-Thr-Asp-Ser-Phe + Val-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Oxidized insulin B-chain + H2O | cleavage sites | Cavia porcellus | Hydrolyzed oxidized insulin B-chain | - |
? | |
| 3.4.23.34 | Oxidized insulin B-chain + H2O | cleavage sites | Homo sapiens | Hydrolyzed oxidized insulin B-chain | - |
? | |
| 3.4.23.34 | Oxidized insulin B-chain + H2O | cleavage sites | Rattus norvegicus | Hydrolyzed oxidized insulin B-chain | - |
? | |
| 3.4.23.34 | Oxidized insulin B-chain + H2O | cleavage sites | Oryctolagus cuniculus | Hydrolyzed oxidized insulin B-chain | - |
? | |
| 3.4.23.34 | Porcine renin substrate + H2O | i.e. angiotensinogen fragment 1-14 acetate salt or acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser, cleavage site: Leu-Leu | Cavia porcellus | Acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Leu-Val-Tyr-Ser | - |
? | |
| 3.4.23.34 | Porcine renin substrate + H2O | i.e. angiotensinogen fragment 1-14 acetate salt or acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser, cleavage site: Leu-Leu | Oryctolagus cuniculus | Acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Leu-Val-Tyr-Ser | - |
? | |
| 3.4.23.34 | Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Cavia porcellus | Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
| 3.4.23.34 | Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Homo sapiens | Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
| 3.4.23.34 | Pro-Thr-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Cavia porcellus | Pro-Thr-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
| 3.4.23.34 | renin + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Substance P + H2O | i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, best peptide substrate, cleavage site: Phe-Phe | Cavia porcellus | Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Substance P + H2O | i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, best peptide substrate, cleavage site: Phe-Phe | Homo sapiens | Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Substance P + H2O | i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, best peptide substrate, cleavage site: Phe-Phe | Rattus norvegicus | Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Substance P + H2O | i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, best peptide substrate, cleavage site: Phe-Phe | Oryctolagus cuniculus | Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2 | - |
? | |
| 3.4.23.34 | Substance P(1-9) + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Substance P(2-11) + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Substance P(3-11) + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Substance P(4-11) + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Tachykinins + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | Tachykinins + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.23.34 | Tachykinins + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.23.34 | Tachykinins + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.23.34 | [His10]-substance P + H2O | - |
Cavia porcellus | ? | - |
? | |
| 3.4.23.34 | [Tyr8]-substance P + H2O | - |
Cavia porcellus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.34 | dimer | - |
Homo sapiens |
| 3.4.23.34 | dimer | - |
Rattus norvegicus |
| 3.4.23.34 | dimer | - |
Oryctolagus cuniculus |
| 3.4.23.34 | dimer | SDS-PAGE, reducing conditions | Cavia porcellus |
| 3.4.23.34 | dimer | procathepsin E, SDS-PAGE, reducing conditions | Cavia porcellus |
| 3.4.23.34 | dimer | 2 * 39000, SDS-PAGE, reducing conditions | Cavia porcellus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.23.34 | More | current names and systematic | Cavia porcellus |
| 3.4.23.34 | More | current names and systematic | Homo sapiens |
| 3.4.23.34 | More | current names and systematic | Rattus norvegicus |
| 3.4.23.34 | More | current names and systematic | Oryctolagus cuniculus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.34 | 37 | - |
assay at | Cavia porcellus |
| 3.4.23.34 | 37 | - |
assay at | Homo sapiens |
| 3.4.23.34 | 37 | - |
assay at | Rattus norvegicus |
| 3.4.23.34 | 37 | - |
assay at | Oryctolagus cuniculus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.34 | 0.13 | - |
big ET-1 | - |
Homo sapiens | |
| 3.4.23.34 | 0.13 | - |
big ET-1 | pH 4.3 | Cavia porcellus | |
| 3.4.23.34 | 0.74 | - |
Substance P(4-11) | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 10 | - |
Eledoisin | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 13 | - |
Porcine renin substrate | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 18 | - |
acidic fibroblast growth factor | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 19 | - |
Acetyl-substance P(2-11) | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 22 | - |
Substance P(2-11) | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 23 | - |
[His10]-substance P | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 26 | - |
Acetyl-substance P | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 27 | - |
Pro-Thr-Glu-Phe-(4-nitro)Phe-Arg-Leu | pH 3.1 | Cavia porcellus | |
| 3.4.23.34 | 40 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | - |
Homo sapiens | |
| 3.4.23.34 | 40 | - |
[Tyr8]-substance P | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 40 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | [Tyr8]-substance P, pH 5 | Cavia porcellus | |
| 3.4.23.34 | 42 | - |
Neurokinin A | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 47 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | - |
Homo sapiens | |
| 3.4.23.34 | 47 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | substance P | Cavia porcellus | |
| 3.4.23.34 | 58 | - |
Acetyl-substance P(3-11) | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 64 | - |
Arg-modified substance P | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 70 | - |
Substance P(3-11) | pH 5 | Cavia porcellus | |
| 3.4.23.34 | 75 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5 | Cavia porcellus | |
| 3.4.23.34 | 135 | - |
Lys-Pro-Ala-Glu-Phe-(4-nitro)Phe-Arg-Leu | pH 3.1 | Cavia porcellus | |
| 3.4.23.34 | 170 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5 | Cavia porcellus | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.34 | additional information | - |
monomeric cathepsin E is much less stable in weakly alkaline solution than dimeric form | Cavia porcellus |
| 3.4.23.34 | additional information | - |
monomeric cathepsin E is much less stable in weakly alkaline solution than dimeric form | Homo sapiens |
| 3.4.23.34 | additional information | - |
monomeric cathepsin E is much less stable in weakly alkaline solution than dimeric form | Rattus norvegicus |
| 3.4.23.34 | additional information | - |
monomeric cathepsin E is much less stable in weakly alkaline solution than dimeric form | Oryctolagus cuniculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.34 | ATP | at weakly acidic and neutral pH | Cavia porcellus | |
| 3.4.23.34 | ATP | at weakly acidic and neutral pH | Homo sapiens | |
| 3.4.23.34 | ATP | at weakly acidic and neutral pH | Rattus norvegicus | |
| 3.4.23.34 | ATP | at weakly acidic and neutral pH | Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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