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Literature summary extracted from

  • Kainuma, K.; Ookura, T.; Kawamura, Y.
    Purification and characterization of protein disulfide isomerase from soybean (1995), J. Biochem., 117, 208-215.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.4.1 Ca2+ 1 mM, 40% inhibition Glycine max
5.3.4.1 E-64 0.01 mM, 11% inhibition after treatment with 0.01 mM DTT. No inhibition without DTT Glycine max
5.3.4.1 iodoacetate 1 mM, almost complete inhibition after reduction with 0.01 mM DTT. No inhibition in absence of DTT Glycine max
5.3.4.1 Mg2+ 1 mM, 20% inhibition Glycine max
5.3.4.1 Zn2+ 1 mM, 70% inhibition Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.4.1 63000
-
 2 * 63000, SDS-PAGE Glycine max
5.3.4.1 120000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
5.3.4.1 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.4.1
-
 Glycine max

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.4.1 additional information
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.4.1 Proteins native, reduced or with wrong disulfide bonds Glycine max Proteins with correct disulfide bonds ?
5.3.4.1 Proteins incorrectly disulfide-linked Bowman Birk soybean trypsin Glycine max Proteins with correct disulfide bonds ?
5.3.4.1 Proteins incorrectly disulfide-linked lysozyme Glycine max Proteins with correct disulfide bonds ?
5.3.4.1 Proteins incorrectly disulfide-linked RNAse Glycine max Proteins with correct disulfide bonds ?

Subunits

EC Number Subunits Comment Organism
5.3.4.1 dimer 2 * 63000, SDS-PAGE Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.4.1 50
-
 pH 7.5, 1 h, stable up to Glycine max
5.3.4.1 60
-
 pH 7.5, 1 h, about 45% loss of activity Glycine max
5.3.4.1 80
-
 pH 7.5, 1 h, about 70% loss of activity Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.4.1 8
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.4.1 7 9 pH 7: about 50% of maximal activity, pH 9: about 60% of maximal activity Glycine max

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.4.1 10
-
 1 h, 0°C or 37°C, highest stability Glycine max