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Literature summary extracted from

  • Davidson, R.; Gertler, A.; Hofmann, T.
    Aspergillus oryzae acid proteinase. Purification and properties, and formation of pi-chymotrypsin (1975), Biochem. J., 147, 45-53.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.18 0.1
-
 Trypsinogen 35°C, pH 3.5 Aspergillus oryzae
3.4.23.18 0.18
-
 chymotrypsinogen 35°C, pH 3.5 Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.18 additional information
-
 amino acid composition Aspergillus oryzae
3.4.23.18 39400
-
 Aspergillus oryzae, sedimentation equilibrium meniscus depletion method Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.18 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.23.18 glycoprotein 1-2% Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.18 from crude powder preparation Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.18 Benzyloxycarbonyl-Ala-Ala-Phe-Phe 3-(4-pyridyl)propyl ester + H2O
-
 Aspergillus oryzae Benzyloxycarbonyl-Ala-Ala-Phe + Phe 3-(4-pyridyl)propyl ester
-
 ?
3.4.23.18 Benzyloxycarbonyl-His-Phe-Phe ethyl ester + H2O
-
 Aspergillus oryzae Benzyloxycarbonyl-His-Phe + Phe ethyl ester
-
 ?
3.4.23.18 Chymotrypsinogen + H2O cleavage site: Arg15-Ile16 Aspergillus oryzae Pi-Chymotrypsin
-
 ?
3.4.23.18 Chymotrypsinogen + H2O bovine chymotrypsinogen Aspergillus oryzae Pi-Chymotrypsin
-
 ?
3.4.23.18 Trypsinogen + H2O better substrate than chymotrypsinogen Aspergillus oryzae ?
-
 ?
3.4.23.18 Trypsinogen + H2O bovine (trypsinogen) Aspergillus oryzae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.18 35
-
 assay at Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.18 1.14
-
 chymotrypsinogen 35°C, pH 3.5 Aspergillus oryzae
3.4.23.18 11.3
-
 Trypsinogen 35°C, pH 3.5 Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.18 3.2 3.6 chymotrypsin, 25°C Aspergillus oryzae