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Literature summary extracted from

  • Hillson, D.A.; Freedman, R.B.
    Resolution of protein disulphide-isomerase and glutathione-insulin transhydrogenase activities by covalent chromatography (1980), Biochem. J., 191, 373-388.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.4.1 Cd2+
-
 Bos taurus
5.3.4.1 iodoacetate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.4.1 additional information
-
 additional information Km for scrambled ribonuclease: 0.023 mg/ml Bos taurus
5.3.4.1 0.0054
-
 dithiothreitol
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.4.1 57000
-
 x * 57000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.2 Bos taurus
-
-
-
5.3.4.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.4.1 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.2 liver
-
 Bos taurus
-
5.3.4.1 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.4.1 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.2 additional information glutathione-insulin transhydrogenase EC 1.8.4.2 and protein disulfide-isomerase EC 5.3.4.1 activities are not both catalyzed by a single enzyme species Bos taurus ?
-
 ?
5.3.4.1 Proteins native, reduced or with wrong disulfide bonds Bos taurus Proteins with correct disulfide bonds ?
5.3.4.1 Proteins cosubstrate dithiothreitol can be replaced by GSH, cysteamine, 2-mercaptoethanol, thioglycollic acid or L-Cys, but at significantly higher concentrations Bos taurus Proteins with correct disulfide bonds ?
5.3.4.1 ribonuclease + dithiothreitol
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.4.1 ? x * 57000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
1.8.4.2 More glutathione-insulin transhydrogenase EC 1.8.4.2 and protein disulfide-isomerase EC 5.3.4.1 activities are not both catalyzed by a single enzyme species Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.4.1 7.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.4.1 7.2 8 activities are greater than 50% in this range Bos taurus