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Literature summary extracted from
- Purification of prostaglandin A isomerase from rabbit serum (1974), Prep. Biochem., 4, 257-271.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.9 | 5,5'-dithiobis(2-nitrobenzoate) | no effect at 1 mM | Oryctolagus cuniculus |  |
| 5.3.3.9 | NEM | 1 mM, 90% inhibition | Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.9 | 0.054 | - |
prostaglandin A1 | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.9 | 28000 | - |
- |
Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.9 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.3.9 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.3.9 | serum | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.9 | 0.139 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.9 | (13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid | i.e. prostaglandin A1 | Oryctolagus cuniculus | (13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid | the product is prostaglandin C1, which is unstable under mild alkaline conditions and isomerizes to prostaglandin B1 | ? | |
| 5.3.3.9 | prostaglandin A1 | - |
Oryctolagus cuniculus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.9 | 55 | - |
- |
Oryctolagus cuniculus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.9 | 30 | 75 | about 30% of maximal activity at 30°C and 75°C | Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.9 | 60 | - |
10 min, rather stable at | Oryctolagus cuniculus |
| 5.3.3.9 | 70 | - |
10 min, 50% loss of activity | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.9 | 8 | 9 | - |
Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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