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Literature summary extracted from

  • Zhou, Y.; Lindberg, I.
    Purification and characterization of the prohormone convertase PC1(PC3) (1993), J. Biol. Chem., 268, 5615-5623.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.75
-
 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.75 D-Tyr-Ala-Lys-Arg-CH2Cl
-
 Mus musculus
3.4.21.75 EDTA
-
 Mus musculus
3.4.21.75 p-chloromercuribenzenesulfonic acid
-
 Mus musculus
3.4.21.75 Pro-Gly-Lys-Arg-CH2Cl
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.75 Ca2+ required Mus musculus
3.4.21.75 Ca2+ 5 mM Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.75 additional information Mus musculus role in processing of proenkephalin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.75 Mus musculus
-
 expression in Chinese hamster ovary cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.75
-
 Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.75 additional information
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
3.4.21.75 -10°C, 10% glycerol, 90% loss of activity after 1 week Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.75 Carboxybenzyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus ?
-
 ?
3.4.21.75 additional information synthetic tripeptide substrates containing only pairs of basic amino acids are not well cleaved Mus musculus ?
-
 ?
3.4.21.75 additional information the term -+- depicts the point of cleavage, the enzyme prefers substrates with an arginine 4-amino acid amino-terminal to the cleavage site Mus musculus ?
-
 ?
3.4.21.75 additional information role in processing of proenkephalin Mus musculus ?
-
 ?
3.4.21.75 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus ?
-
 ?
3.4.21.75 tert-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.75 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.75 5.5 6.5
-
 Mus musculus