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Literature summary extracted from

  • Scherf, U.; Buckel, W.
    Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA DELTA3-DELTA2-isomerase from Clostridium aminobutyricum (1993), Eur. J. Biochem., 215, 421-429.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.120 additional information enzyme does not require ATP, MgCl2, and Ti(III)citrate Clostridium aminobutyricum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.120 additional information enzyme is not inactivated by 5 mM 4-nitrophenol, 5 mM chloramphenicol, and 5 mM hydroxylamine Clostridium aminobutyricum
5.3.3.3 Cu2+
-
 Clostridium aminobutyricum
5.3.3.3 additional information not inhibited by Fe2+, Mn2+, Co2+, Ni2+, Ca2+, Mg2+, Zn2+ Clostridium aminobutyricum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.120 0.05
-
 4-hydroxybutanoyl-CoA pH 9.0, 25°C Clostridium aminobutyricum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.120 Iron 16 mol per mol of enzyme, plus 14 mol sulfur per mol of enzyme Clostridium aminobutyricum
5.3.3.3 Fe-S 16 mol Fe and 14 mol S per mol of enzyme, iron-sulfur protein, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium aminobutyricum
5.3.3.3 additional information no activation by Fe2+, Mn2+, Co2+, Ni2+, Ca2+, Mg2+, Zn2+ Clostridium aminobutyricum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.120 56000
-
 4 * 56000, SDS-PAGE Clostridium aminobutyricum
4.2.1.120 232000
-
 gel filtration Clostridium aminobutyricum
5.3.3.3 56000
-
 4 * 56000, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, SDS-PAGE Clostridium aminobutyricum
5.3.3.3 232000
-
 bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, gel filtration Clostridium aminobutyricum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.120 Clostridium aminobutyricum
-
-
-
4.2.1.150 Clostridium aminobutyricum
-
-
-
5.3.3.3 Clostridium aminobutyricum Q03161
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.120 exposure to air results in initial activation followed by irreversible inactivation Clostridium aminobutyricum
5.3.3.3 oxygen irreversibly inactivates Clostridium aminobutyricum

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.120 using anaerobic conditions Clostridium aminobutyricum
5.3.3.3 bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium aminobutyricum

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.120 4-hydroxybutanoyl-CoA = (E)-but-2-enoyl-CoA + H2O cleavage is achieved by a FAD-dependent oxidation of 4-hydroxybutanoyl-CoA to 4-hydroxycrotonyl-CoA. In a second step, the hydroxyl group is substituted by a hydride derived from the now reduced FAD in an SN2' reaction leading to vinylacetyl-CoA. Isomerization yields crotonyl-CoA Clostridium aminobutyricum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.120 12.5
-
 pH 9.0, 25°C Clostridium aminobutyricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.120 4-hydroxybutanoyl-CoA
-
 Clostridium aminobutyricum vinylacetyl-CoA + H2O
-
 r
4.2.1.120 additional information no substrate: cyclopropylcarboxyl-CoA Clostridium aminobutyricum ?
-
 ?
4.2.1.150 crotonyl-CoA + H2O
-
 Clostridium aminobutyricum 3-hydroxybutyryl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.120 tetramer 4 * 56000, SDS-PAGE Clostridium aminobutyricum
5.3.3.3 tetramer 4 * 56000, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, SDS-PAGE Clostridium aminobutyricum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.150 3-hydroxyacyl-CoA dehydratase
-
 Clostridium aminobutyricum
4.2.1.150 crotonase
-
 Clostridium aminobutyricum

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.120 FAD 2 mol per mol of enzyme Clostridium aminobutyricum
5.3.3.3 FAD 2 mol per mol of enzyme, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium aminobutyricum