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Literature summary extracted from
- Purification and characterization of tissue plasminogen activator secreted by human embryonic lung diploid fibroblasts, IMR-90 cells [published erratum appears in Agric Biol Chem 1991 Jun;55(6):A10] (1991), Agric. Biol. Chem., 55, 1225-1232.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.68 | Cd2+ | inhibition of amidolytic activity | Homo sapiens |  |
| 3.4.21.68 | Co2+ | inhibition of amidolytic activity | Homo sapiens | |
| 3.4.21.68 | Cu2+ | inhibition of amidolytic activity | Homo sapiens | |
| 3.4.21.68 | DFP | - |
Homo sapiens | |
| 3.4.21.68 | Gabexate | - |
Homo sapiens | |
| 3.4.21.68 | Hg2+ | inhibition of amidolytic activity | Homo sapiens | |
| 3.4.21.68 | leupeptin | - |
Homo sapiens | |
| 3.4.21.68 | mesilate | - |
Homo sapiens | |
| 3.4.21.68 | Ni2+ | inhibition of amidolytic activity | Homo sapiens | |
| 3.4.21.68 | p-aminobenzamidine | - |
Homo sapiens | |
| 3.4.21.68 | Zn2+ | inhibition of amidolytic activity | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.68 | 0.000017 | - |
Peptide S-2288 | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | 69000 | 72000 | human, gel filtration | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.68 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.68 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.68 | fibroblast | embryonic lung diploid cells, IMR-90 cells, ATCC, CCL186 | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | Peptide S-2288 + H2O | chromogenic substrate | Homo sapiens | ? | - |
? | |
| 3.4.21.68 | plasminogen + H2O | - |
Homo sapiens | plasmin + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | monomer | 1 * 64000-68000, human, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | 41 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | 95 | - |
5 min, stable | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | 9 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | 7 | 10 | 7: about 85% of activity maximum, 10: about 80% of activity maximum | Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | 5 | 10 | stable | Homo sapiens |
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