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Literature summary extracted from
- The purification and properties of an extracellular protease from Aspergillus oryzae NRRL 2160 (1973), Biochim. Biophys. Acta, 304, 505-512.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.63 | 4-nitrophenyl acetate | increasing concentrations of the ester inhibit casein hydrolysis | Aspergillus oryzae |  |
| 3.4.21.63 | DFP | - |
Aspergillus oryzae | |
| 3.4.21.63 | PMSF | - |
Aspergillus oryzae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.63 | extracellular | enzyme produced in shake cultures is secreted in the stationary phase when cells are grown on nutrient broth, and in early growth on defined salts | Aspergillus oryzae | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | 25000 | - |
Aspergillus oryzae, gel filtration | Aspergillus oryzae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.63 | Aspergillus oryzae | - |
NRRL 2160 | - |
| 3.4.21.63 | Aspergillus oryzae NRRL 2160 | - |
NRRL 2160 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.63 | NRRL 2160 | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.63 | 4-nitrophenyl acetate + H2O | - |
Aspergillus oryzae | 4-nitrophenol + acetate | - |
? | |
| 3.4.21.63 | 4-nitrophenyl acetate + H2O | - |
Aspergillus oryzae NRRL 2160 | 4-nitrophenol + acetate | - |
? | |
| 3.4.21.63 | casein + H2O | - |
Aspergillus oryzae | hydrolyzed casein | - |
? | |
| 3.4.21.63 | casein + H2O | - |
Aspergillus oryzae NRRL 2160 | hydrolyzed casein | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.63 | More | protein is not composed of subunits | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | 7.5 | 8 | N-carbobenzoxyglycine 4-nitrophenyl ester | Aspergillus oryzae |
| 3.4.21.63 | 8 | 8.5 | casein | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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