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Literature summary extracted from

  • Nakagawa, Y.
    Alkaline proteinases from Aspergillus (1970), Methods Enzymol., 19, 581-591.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.63 DFP
-
 Aspergillus flavus
3.4.21.63 DFP
-
 Aspergillus oryzae
3.4.21.63 DFP
-
 Aspergillus sydowii
3.4.21.63 additional information cysteine; EDTA; KCN; not: sulfhydryl reagents; PCMB Aspergillus flavus
3.4.21.63 additional information cysteine; EDTA; KCN; not: sulfhydryl reagents; PCMB Aspergillus oryzae
3.4.21.63 additional information cysteine; EDTA; KCN; not: sulfhydryl reagents; PCMB Aspergillus sydowii
3.4.21.63 N-bromosuccinimide
-
 Aspergillus flavus
3.4.21.63 N-bromosuccinimide
-
 Aspergillus oryzae
3.4.21.63 N-bromosuccinimide
-
 Aspergillus sydowii
3.4.21.63 Potato inhibitor
-
 Aspergillus oryzae
3.4.21.63 Potato inhibitor
-
 Aspergillus sydowii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.63 extracellular
-
 Aspergillus flavus
-
-
3.4.21.63 extracellular
-
 Aspergillus oryzae
-
-
3.4.21.63 extracellular
-
 Aspergillus sydowii
-
-
3.4.21.63 extracellular
-
 Aspergillus sojae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.63 18000
-
 Aspergillus flavus Aspergillus flavus
3.4.21.63 25500
-
 Aspergillus sojae Aspergillus sojae

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.63 Aspergillus flavus
-
-
-
3.4.21.63 Aspergillus oryzae
-
-
-
3.4.21.63 Aspergillus sojae
-
-
-
3.4.21.63 Aspergillus sydowii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.63 additional information Aspergillus sojae enzyme contains no sugar, Aspergillus flavus alkaline proteinase contains 1 mol ribose per mol of protein Aspergillus flavus
3.4.21.63 additional information Aspergillus sojae enzyme contains no sugar, Aspergillus flavus alkaline proteinase contains 1 mol ribose per mol of protein Aspergillus sojae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.63
-
 Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.63 additional information
-
-
 Aspergillus flavus
3.4.21.63 additional information
-
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.63 Hemoglobin + H2O
-
 Aspergillus flavus Hydrolyzed hemoglobin
-
 ?
3.4.21.63 Hemoglobin + H2O
-
 Aspergillus oryzae Hydrolyzed hemoglobin
-
 ?
3.4.21.63 Hemoglobin + H2O
-
 Aspergillus sydowii Hydrolyzed hemoglobin
-
 ?
3.4.21.63 Hemoglobin + H2O
-
 Aspergillus sojae Hydrolyzed hemoglobin
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.63 35
-
 pH 7.5, highest stability Aspergillus sydowii
3.4.21.63 55
-
 pH 7.5, 10 min, complete inactivation after incubation in 0.01 M sodium phosphate, Aspergillus sydowi Aspergillus sydowii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.63 additional information
-
 pI: 5.1 (Aspergillus sojae) Aspergillus sojae
3.4.21.63 9 10 Aspergillus sojae Aspergillus sojae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.63 7.5
-
 35°C, highest stability Aspergillus sydowii