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Literature summary extracted from

  • Bruenger, E.; Chayet, L.; Rilling, H.C.
    Isopentenyl pyrophosphate isomerase:dimetylallyl pyrophosphate isomerase: isolation from Claviceps sp. SD 58 and Comparison to the mammalian enzyme (1986), Arch. Biochem. Biophys., 248, 620-625.
    View publication on PubMed

General Stability

EC Number General Stability Organism
5.3.3.2 high susceptibility to proteolysis Claviceps sp.
5.3.3.2 unstable in dilute solutions Claviceps sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.2 2,3-cis,6,7-trans-farnesyl diphosphate
-
 Gallus gallus
5.3.3.2 3-methyl-5-oxo-hexyl diphosphate
-
 Gallus gallus
5.3.3.2 3-methyl-5-oxo-hexyl diphosphate ethylene glycol acetal
-
 Gallus gallus
5.3.3.2 dimethylallyl diphosphate
-
 Gallus gallus
5.3.3.2 diphosphate
-
 Claviceps sp.
5.3.3.2 diphosphate
-
 Gallus gallus
5.3.3.2 farnesyl diphosphate
-
 Claviceps sp.
5.3.3.2 farnesyl diphosphate
-
 Gallus gallus
5.3.3.2 geranyl diphosphate
-
 Claviceps sp.
5.3.3.2 geranyl diphosphate
-
 Gallus gallus
5.3.3.2 iodoacetic acid
-
 Claviceps sp.
5.3.3.2 Octyl diphosphate
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.2 0.0054
-
 isopentenyl diphosphate
-
 Claviceps sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.3.2 Mg2+
-
 Gallus gallus
5.3.3.2 Mg2+ Mn2+ or Mg2+ required Claviceps sp.
5.3.3.2 Mg2+ maximal activation at 0.2 mM Claviceps sp.
5.3.3.2 Mn2+
-
 Gallus gallus
5.3.3.2 Mn2+ Mn2+ or Mg2+ required Claviceps sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.2 35000
-
 gel filtration Claviceps sp.

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Claviceps sp.
-
-
-
5.3.3.2 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.2
-
 Claviceps sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.3.2 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.3.2 3000
-
-
 Claviceps sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 isopentenyl diphosphate
-
 Gallus gallus dimethylallyl diphosphate
-
 ?
5.3.3.2 isopentenyl diphosphate
-
 Claviceps sp. dimethylallyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.3.2 monomer 1 * 35000, SDS-PAGE Claviceps sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.3.2 37
-
 half-life in dilute solution: 30 min. In more concentrated solutions of the protein or dilute enzyme in the presence of 0.01% bovine serum albumin, little loss of activity after 1 h Claviceps sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.2 6 8.5 in presence of Mg2+. A sharper maximum is observed in presence of Mn2+ Claviceps sp.