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Literature summary extracted from

  • Jones, B.L.; Porter, J.W.
    Enzymatic synthesis of phytoene (1985), Methods Enzymol., 110, 209-220.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.32 ATP stimulates Solanum lycopersicum
2.5.1.32 dithiothreitol activates Solanum lycopersicum
2.5.1.32 NADP+ weak stimulation Solanum lycopersicum
5.3.3.2 dithiothreitol activates Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.32 iodoacetamide
-
 Solanum lycopersicum
2.5.1.32 NEM
-
 Solanum lycopersicum
2.5.1.32 p-hydroxymercuribenzoate
-
 Solanum lycopersicum
5.3.3.2 dimethylallyl diphosphate
-
 Solanum lycopersicum
5.3.3.2 geranyl diphosphate
-
 Solanum lycopersicum
5.3.3.2 iodoacetamide
-
 Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.32 0.026
-
 geranylgeranyl diphosphate
-
 Solanum lycopersicum
5.3.3.2 0.0057
-
 isopentenyl diphosphate
-
 Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.32 plastid
-
 Solanum lycopersicum 9536
-
5.3.3.2 chromoplast
-
 Solanum lycopersicum 9509
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.32 Mg2+ required Solanum lycopersicum
2.5.1.32 Mn2+
-
 Solanum lycopersicum
5.3.3.2 Mg2+ Mn2+ or Mg2+ required Solanum lycopersicum
5.3.3.2 Mn2+ Mn2+ or Mg2+ required Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.32 200000
-
 gel filtration Solanum lycopersicum
5.3.3.2 34000
-
 gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.2 additional information Solanum lycopersicum enzyme in the pathway of conversion of isopentenyl diphosphate to phytoene ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.32 Solanum lycopersicum
-
-
-
5.3.3.2 Solanum lycopersicum
-
 tomato
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.32
-
 Solanum lycopersicum
5.3.3.2
-
 Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.32 fruit
-
 Solanum lycopersicum
-
5.3.3.2 fruit
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.3.2 additional information
-
-
 Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate
-
 Solanum lycopersicum prephytoene diphosphate + diphosphate
-
 ?
5.3.3.2 isopentenyl diphosphate
-
 Solanum lycopersicum dimethylallyl diphosphate
-
 ?
5.3.3.2 additional information enzyme in the pathway of conversion of isopentenyl diphosphate to phytoene Solanum lycopersicum ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.32 ATP stimulates Solanum lycopersicum