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Literature summary extracted from

  • Cromlish, J.A.; Seidah, N.G.; Marcinkiewicz, M.; Hamelin, J.; Jonson, D.A.; Chretien, M.
    Human pituitary tryptase: molecular forms, NH2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates (1987), J. Biol. Chem., 262, 1363-1373.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.59 antipain
-
 Homo sapiens
3.4.21.59 CaCl2
-
 Homo sapiens
3.4.21.59 D-Tyr-Glu-Phe-Lys-Arg-CH2Cl
-
 Homo sapiens
3.4.21.59 diisopropyl fluorophosphate
-
 Homo sapiens
3.4.21.59 DTNB
-
 Homo sapiens
3.4.21.59 HgCl2
-
 Homo sapiens
3.4.21.59 leupeptin
-
 Homo sapiens
3.4.21.59 N-tosyl-L-Lys chloromethyl ketone
-
 Homo sapiens
3.4.21.59 NaCl reversible at 1 M Homo sapiens
3.4.21.59 p-chloromercuribenzoate
-
 Homo sapiens
3.4.21.59 PMSF
-
 Homo sapiens
3.4.21.59 ZnSO4
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.59 0.0138
-
 Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens
3.4.21.59 0.0148
-
 Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide
-
 Homo sapiens
3.4.21.59 0.0409
-
 Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens
3.4.21.59 0.323
-
 benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.59 110000
-
 human, gel filtration in presence of 0.3 M NaCl Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.59 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.59 glycoprotein some of the MW heterogeneity of the multiple forms results from N-linked glycosylation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.59 multiple forms Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.59 mast cell present in pituitary connective tissue Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.21.59 -20°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.59 Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide + H2O
-
 Homo sapiens Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.59 benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Ala-L-Leu-L-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.59 Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide + H2O
-
 Homo sapiens Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.59 Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide + H2O
-
 Homo sapiens Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.59 additional information pituitary enzyme has a preference for cleaving COOH-terminal to arginine or lysine residues which are preceded by a proline residue 4 or 6 residues NH2-terminal to the site of cleavage Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.59 tetramer 4 * 32400-36300, human, SDS-PAGE, various enzyme forms Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.59 1520
-
 Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide
-
 Homo sapiens
3.4.21.59 1870
-
 benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin
-
 Homo sapiens
3.4.21.59 2040
-
 Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens
3.4.21.59 3290
-
 Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens