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Literature summary extracted from
- Metabolism of resorcinylic compounds by bacteria. Purification and properties of acetylpyruvate hydrolase from Pseudomonas putida 01 (1975), J. Biol. Chem., 250, 3826-3830.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.7.1.6 | Cu2+ | - |
Pseudomonas putida |  |
| 3.7.1.6 | oxalacetate | competitive inhibition, 0.2-1 mM | Pseudomonas putida | |
| 3.7.1.6 | oxalate | competitive inhibition, 2-6 mM | Pseudomonas putida | |
| 3.7.1.6 | pyruvate | competitive inhibition, 2-10 mM | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.6 | 0.1 | - |
Acetylpyruvate | - |
Pseudomonas putida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.7.1.6 | Ca2+ | 0.33 mM | Pseudomonas putida | |
| 3.7.1.6 | Co2+ | 0.33 mM | Pseudomonas putida | |
| 3.7.1.6 | Mg2+ | 0.33 mM | Pseudomonas putida | |
| 3.7.1.6 | Mn2+ | 0.33 mM | Pseudomonas putida | |
| 3.7.1.6 | Zn2+ | 0.33 mM | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.6 | 38000 | - |
SDS-PAGE | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.6 | acetylpyruvate + H2O | Pseudomonas putida | plays a role in the orcinol catabolism | acetate + pyruvate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.7.1.6 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.7.1.6 | - |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.6 | 0.8 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.6 | acetylpyruvate + H2O | plays a role in the orcinol catabolism | Pseudomonas putida | acetate + pyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.7.1.6 | monomer | 1 * 38000, SDS-PAGE | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.6 | 7.4 | - |
- |
Pseudomonas putida |
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Release 2023.1 (January 2023)
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