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Literature summary extracted from

  • Minamikawa, T.; Jayasankar, N.P.; Bohm, B.A.; Taylor, I.E.P.; Towers, G.H.N.
    An inducible hydrolase from Aspergillus niger, acting on carbon-carbon bonds, for phlorrhizin and other C-acylated phenols (1970), Biochem. J., 116, 889-897.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.4 Ag+ 0.02 mM Aspergillus niger
3.7.1.4 Al2(SO4)3 1 mM Aspergillus niger
3.7.1.4 Cd2+ 1 mM Aspergillus niger
3.7.1.4 Co2+ 1 mM Aspergillus niger
3.7.1.4 Cu2+ 1 mM Aspergillus niger
3.7.1.4 Fe3+ 1 mM Aspergillus niger
3.7.1.4 Hg2+ 0.02 mM Aspergillus niger
3.7.1.4 L-cysteine in presence of p-chloromercuribenzoate, 0.05 mM Aspergillus niger
3.7.1.4 Na2MoO4 1 mM Aspergillus niger
3.7.1.4 Na2WO4 1 mM Aspergillus niger
3.7.1.4 p-chloromercuribenzoate 0.05 mM Aspergillus niger
3.7.1.4 Zn2+ 0.2 mM Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.4 0.15
-
2-chloro-1-(3-methylphenyl)ethanone
-
Aspergillus niger
3.7.1.4 0.3 0.4 phloretin
-
Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.4 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.4
-
Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.7.1.4 mycelial felt
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.4 3.6
-
-
Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.4 2',3,4',6'-tetrahydroxy-4-methoxydihydrochalcone + H2O
-
Aspergillus niger phloroglucinol + ?
-
?
3.7.1.4 2',3,4',6'-tetrahydroxy-dihydrochalcone + H2O
-
Aspergillus niger phloroglucinol + ?
-
?
3.7.1.4 2',3,4,4',6'-pentahydroxy-dihydrochalcone 2'-beta-D-glucoside + H2O
-
Aspergillus niger phloroglucinol beta-D-glucoside + 3-(3,4-dihydroxyphenyl)propanoic acid
-
?
3.7.1.4 2',4'-dihydroxy-4-methoxydihydrochalcone + H2O
-
Aspergillus niger resorcinol + ?
-
?
3.7.1.4 2',4'-dihydroxypropiophenone + H2O
-
Aspergillus niger resorcinol
-
?
3.7.1.4 2',4,4',6'-tetrahydroxy-dihydrochalcone + H2O
-
Aspergillus niger phloroglucinol + ?
-
?
3.7.1.4 2',4,4'-trihydroxyhydrochalcone
-
Aspergillus niger ?
-
?
3.7.1.4 2',6'-dihydroxy-4,4'-dimethoxydihydrochalcone + H2O
-
Aspergillus niger 3-methoxyresorcinol + ?
-
?
3.7.1.4 3'-methylchloracetophenone + H2O
-
Aspergillus niger ?
-
?
3.7.1.4 chloracetophenone + H2O
-
Aspergillus niger ?
-
?
3.7.1.4 phloretin + H2O
-
Aspergillus niger phloretate + phloroglucinol
-
?
3.7.1.4 phlorhizin + H2O
-
Aspergillus niger 3-(4-hydroxyphenyl)propanoate + arbutin
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.7.1.4 -20
-
after 4 weeks loss of 20% activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.4 9.6
-
-
Aspergillus niger