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Literature summary extracted from

  • Mahuran, D.J.; Angus, R.H.; Braun, C.V.; Sim, S.S.; Schmidt, Jr., D.E.
    Characterization and substrate specificity of fumarylacetoacetate fumarylhydrolase (1977), Can. J. Biochem., 55, 1-8.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.2 0.12
-
 benzoylpyruvate
-
 Bos taurus
3.7.1.2 0.14
-
 6,6-dimethyl-3,5-dioxoheptanoate
-
 Bos taurus
3.7.1.2 0.22
-
 3-phenylpropionopyruvate
-
 Bos taurus
3.7.1.2 0.27
-
 3,5-dioxohexanoate
-
 Bos taurus
3.7.1.2 0.31
-
 butyropyruvate
-
 Bos taurus
3.7.1.2 0.47
-
 Acetopyruvate
-
 Bos taurus
3.7.1.2 0.47
-
 propionopyruvate
-
 Bos taurus
3.7.1.2 0.64
-
 pivalopyruvate
-
 Bos taurus
3.7.1.2 0.89
-
 5-phenyl-3,5-dioxopentanoate
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.2 86000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.7.1.2 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.2 0.35
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.2 3,5-dioxohexanoate + H2O
-
 Bos taurus ?
-
 ?
3.7.1.2 3-phenylpropionopyruvate + H2O
-
 Bos taurus ?
-
 ?
3.7.1.2 5-phenyl-3,5-dioxopentanoate + H2O
-
 Bos taurus ?
-
 ?
3.7.1.2 6,6-dimethyl-3,5-dioxoheptanoate + H2O
-
 Bos taurus ?
-
 ?
3.7.1.2 acetopyruvate + H2O
-
 Bos taurus ?
-
 ?
3.7.1.2 benzoylpyruvate + H2O
-
 Bos taurus pyruvate + benzoate
-
 ?
3.7.1.2 butyropyruvate + H2O
-
 Bos taurus ?
-
 ?
3.7.1.2 pivalopyruvate + H2O
-
 Bos taurus ?
-
 ?
3.7.1.2 propionopyruvate + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.2 dimer 2 * 38000-43000, SDS-PAGE, gel filtration, ultracentrifugation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.2 7.3
-
-
 Bos taurus